摘要
目的研究慢性特发性血小板减少性紫癜（CITP）患者经氟美松或静脉注射用人免疫球蛋白治疗后辅助性T细胞（Th）细胞因子谱的变化。方法对CITP、系统性红斑狼疮伴有血小板减少（SLETP）、再生障碍性贫血（AA）患者及正常对照分别用ELISA及RIA方法测定细胞因子谱。用RT—PCR检测辅助性T细胞相关的转录因子c-Mar及T-bet。结果经氟美松和／或静脉注射人免疫球蛋白治疗的CITP患者均取得了良好的反应，体内IL-2水平显著低于正常对照。但是Th2细胞因子IL-4水平显著高于正常对照。用PHA对CITP患者外周血单个核细胞（PBMC）刺激后，IL-13的分泌量明显增高，而正常对照PBMC受刺激后IL-13水平很低。结论CITP患者经氟美松／静脉注射人免疫球蛋白治疗后倾向于Th2细胞因子分泌，这可能是患者获得病情缓解的机制之一。
Objective To determine the T helper cell cytokine profile in patients with chronic idiopathic thrombocytopenia purprua （CITP） receiving dexamethasone （DXM） and/or intravenous immunoglobulin （IVIG）. Methods By means of ELISA and RIA, cytokine profile in patients with CITP, systemic lupus erythematosus with thrombocytopenia （SLE-TP） and aplastic anemia （AA）, and normal controls were assayed respectively. In the meantime, RT-PCR was performed to explore the T helper cellassociated transcript factors, e.g. c-Mar and T-bet. Results After DXM and/or IVIG treatment, all the （TITP patients showed a good response and IL-2 level in CITP patients was significant lower than that in the normal controls, however, Th2-1ike cytokine, IL-4 was significant higher in patients than in normal controls. On stimulation with PHA, significant higher secretion of IL-13 was found in PBMC from patients with CITP, whereas PBMC in normal controls showed very low levels of IL-13. Conclusions After DXM/IVIG treatment, CITP patients skewed to Th2-1ike cytokine pattern, and this might be the mechanism of the amelioration of ITP.
出处
《国际输血及血液学杂志》
CAS
2007年第6期487-490,共4页
International Journal of Blood Transfusion and Hematology
基金
国家自然科学基金（30670900）、教育部高等院校博士点基金（20060023031）和2006年度人事部留学回国人员科技活动择优资助项目（优秀娄）部分瓷助
