摘要
目的:观察PDGF-BB诱导大鼠肾小球系膜细胞增殖过程中氧化与抗氧化系统的改变及炎症介质的分泌变化,并探讨迷迭香酸的干预作用。方法:培养大鼠系膜细胞,以PDGF刺激系膜细胞增殖及迷迭香酸干预。实验设正常对照组、增殖组、单纯迷迭香酸组和迷迭香酸干预组,利用分光法分别测定细胞上清液中超氧化物歧化酶活性和丙二醛含量。应用双抗体夹心ELISA法检测培养上清液中TGF-β1,MCP-1及纤维连结蛋白的蛋白分泌量。结果:经PDGF-BB刺激系膜细胞后超氧化物歧化酶活性降低,丙二醛含量升高;而迷迭香酸干预能促进超氧化物歧化酶活性升高而降低丙二醛的产生。PDGF-BB(20ng/mL)同样刺激肾小球系膜细胞分泌TGF-β1与MCP-1,刺激GMC合成FN蛋白。RA则呈浓度依赖性地抑制肾小球系膜细胞中TGF-β1,MCP-1,FN蛋白分泌。结论:氧自由基、脂质过氧化及炎症介质参与了系膜细胞的增生,而迷迭香酸则能抑制系膜细胞的上述改变,具有抗氧化活性。同时在PDGF-BB诱导的肾小球系膜细胞增殖中,抑制肾球系膜细胞分泌TGF-β1与MCP-1,并能抑制肾小球系膜细胞合成纤维连结蛋白。
Objective To investigate the change of oxidation system and antioxidation system and cytokines production in mesangial cell proliferative process,and further to study the intervention of rosmarinic acid. Methods Rat mesangial cells were cultured and stimulated by PDGF to make mesangial proliferation. The cells were divided into normal control group, proliferation group, simple rosmarinic acid group and rosmarinic acid interfering group. The activity of SOD and the content of MDA in cell supernatants were detected by spectrophotomerty. The levels of the MCP-1, TGF-β1and FN protein in the supernatant were measured by ELISA. Results The activity of SOD was found to be significantly decreased, while the content of MDA increased and the MCP-1, TGF-β1 and FN protein could be induced in rat GMC. Whereas rosmarinic acid could inhibit the oxidation and the protein production of MCP-1, TGF-β1 and FN in GMC induced by PDGF-BB in a dose-dependent manner. Conclusion ROS, lipid peroxidation and cytokines participates in the proliferation of GMC and RA can control the changes of the mesangial cells, showing the activity of antioxidation and down-regulate the secretion of MCP-1, TGF-β1 and FN in rat GMC induced by PDGF-BB.
出处
《实用诊断与治疗杂志》
2007年第12期888-890,共3页
Journal of Practical Diagnosis and Therapy
基金
江苏省科委社会发展基金资助项目(BS2007014)
南京市社会发展基金资助项目(200702082)
