摘要
甜菜碱醛脱氢酶(Betaine aldehyde dehydrogenase,BADH)对非生物胁迫下植物渗透调节物质的合成和积累具有重要作用。分别从异苞滨藜和鞑靼滨藜两种盐生植物中分离到了BADH基因。序列分析表明,BADH全长均为1 507bp,编码501个氨基酸,两种BADH序列具有较高的相似性。甜菜碱醛脱氢酶的克隆为植物的基因转化及其功能分析奠定了基础。
Betaine aldehyde dehydrogenase （BADH）plays a vital role of osmotic adjustment substances synthesize and accumulate by abiotic stresses.The BADH genes were amplified from Atriplex micrantha C. A. Mey and Atriplex tatarica L through PT-PCR technique.sequence analysis shows BADHs both were 1 507bp in full length,and encoding 501 amino acid.The two BADH have high comparability.Cloning of BADH may set up a solid foundation for giving further insight on plant transgenetic and function analysis.
出处
《生物技术通报》
CAS
CSCD
2007年第6期88-91,共4页
Biotechnology Bulletin
关键词
异苞滨藜
鞑靼滨藜
BADH
基因克隆
序列分析
Atriplex micrantha C.A.Mey Atriplex tatarica L. BADH Gene cloning Sequence analysis
