摘要
用不连续密度梯度离心法分离恶性胸液淋巴细胞（MPEL），比较了MPEL与外周血淋巴细胞（PBL）在重组白细胞介素－2（rIL2）诱导前后T细胞亚群的动态变化。结果MPEL中以T淋巴细胞为主，其中OKT8所占比例大于OKT4，PBL中也以T淋巴细胞为主，其中OKT4所占比例大于OKT8；MPEL中，OKT3、OKT4、OKT4／OKT8均较PBL中为低，OKT8则较PBL中为高。经rIL2激活后，MPEL、PBL中T淋巴细胞均明显增加且两者数量上无明显差异，MPEL中OKT4逐渐增多并于10天时超过OKT8（P＜0．001），PBL中OKT4始终多于OKT8。表明恶性胸液患者胸腔局部免疫功能较全身免疫功能更为低下，经rIL2激活可得到一定改善。
Malignant pleural effusion lymphocytes (MPEL) were obtained by centrifugation on discontinuous density gradients for 16 patients with malignant pleural effusion. The T cell subpopulation of them were compared before and after MPEL and peripheral blood lymphocytes (PBL) was activated by recombinant interleukin- 2 (rIL2). It was demonstrated that the T lymphocytes were chief cells and OKT8 were on than OKT4 in MPEL, but OKT4 were more than OKT8 in PBL although the T lymphocytes were also chief cells in PBL. OKT3、OKT4 and OKT4/OKT8 of MPEL were little than that of PBL. The numbers of T lymphocytes of tham were increased and no difference between the MPEL ho PBL after they activated by rIL2. OKT4 were increased along with prolongation of cultural the and exceed the OKT8 at tenth day in MPEL -activated, but OKT4 were more than OKT8 in PBL-activated all along.This shows that local immunologic function within pleural cavity of patients with malignant pleural effusion was mere suppressant than that of general immunologic function, but the state could be improved by using rIL2.
出处
《兰州医学院学报》
1997年第2期5-7,共3页
Journal of Lanzhou Medical College
关键词
胸膜积液
恶性
重组
白细胞介素2
T细胞
肿瘤
Pleural effusion, Malignant
T cell subpopulation
Recombinant interleukin-2
