摘要
背景与目的:比较三价铬(CrCl3)和六价铬(K2Cr2O7)对大鼠长期慢性毒性。材料与方法:采用90d喂养实验。Wistar大鼠按10%LD50、6%LD50、2%LD50分为高、中、低3个剂量组,同时设立阴性对照,染毒90d后检测血清学指标、脏/体比及组织病理学检测。结果:CrCl3高剂量组CHO、HDL、TG、Glu与对照组相比显著降低(P<0.05),其余指标均无显著性差异(P<0.05)。K2Cr2O7高剂量组大鼠体重随着染毒剂量的增加而下降,与对照组相比降低显著(P<0.05);高剂量组白细胞计数与对照组相比有显著性升高,淋巴细胞与对照组相比显著降低(P均<0.05);血清学指标检测显示,高剂量组CHO、HDL、TG、Glu与对照组相比显著降低,而ALT、Cr、BUN与对照组相比则显著升高(P均<0.05)。病理组织学检查显示,K2Cr2O7高剂量组肝脏表现为明显的肝窦扩张,淤血及少量渗出性出血,并偶见坏死灶。结论:K2Cr2O7高剂量组对大鼠免疫系统、肝、肾脏有损伤;K2Cr2O7长期慢性毒性明显高于CrCl3。
BACKGROUND ＆ AIM： To evaluate the chronic toxic effects of trivalent and hexavalent chromium to rats. MATERIALS AND METHODS： Groups were set up on the basis of 10% LD50,6% LD50,2% LD50,and the negative control group simultaneously. After ninety-days feeding test, the sermn and histopathological indexes and the ratio of viscera and body were assessed. RESULTS： The CHO, HDL, TG, Glu were significantly decreased in high-dose group of CrCl3 and control group（P 〈0.05） .The body weight gradual decreased with increasing doses of K2Cr2O7, and the difference were significant compared with control group（P 〈 0.05） .The erythrocyte count were significantly higher than that of control group（P 〈 0.05）, and lymphocyte were significantly lower. Sermn ALT, BUN, Cr were raised but HDL, TG, Glu were decreased in the high-dose group of K2Cr2O7 and were significantly higher than that of control group.Pathological the changes in the high-dose group of K2Cr207 showed hepatic sinus dilation and congestion, mild exudative hemorrhage,and focal necrosis was found. CONCLUSION： K2Cr2O7 could damage the immunologic system, liver and kidney. The chronic toxicity of K2Cr2O7 was obviously higher than CrCl3.
出处
《癌变．畸变．突变》
CAS
CSCD
2007年第6期474-478,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
农业部农业行业标准项目(03184)
关键词
铬
慢性毒性
chromium
chronic toxicity
