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FAM3B基因cDNA克隆及其在胃癌细胞系BGC-823的表达 被引量：1

Cloning and expression of FAM3B gene in gastric carcinoma cell line BGC-823
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摘要 目的克隆FAM3B cDNA,构建FAM3B重组表达载体并在胃癌细胞系BGC-823中表达,观察其对细胞系的影响,探索新的肿瘤治疗途径。方法构建pEGFP-N2-FAM3B真核表达质粒,将pEGFP-N2-FAM3B转染人胃癌细胞系BGC-823,研究其对胃癌细胞产生的作用。结果重组pEGFP-N2-FAM3B真核表达质粒构建成功,经pEGFP-N2-FAM3B转染后,荧光显微镜下可见转染的BGC-823细胞有绿色荧光蛋白的表达,转染后的BGC-823细胞生长减慢。结论构建完成真核表达载体pEGFP-N2-FAM3B,重组pEGFP-N2-FAM3B质粒在胃癌细胞系BGC-823细胞内成功表达。 Objective To construct an eukaryotic expression vector of human FAM3B full-length gene, pEGFP-N2-FAM3B, transfect it into gastric cancer cell line BGC-823 and observe its effects on BGC-823, in a search for new approach of tumor therapy. Method The full-length gene sequence of FAM3B （708 bp） was obtained with RT-PCR using human gastric epithelium mRNA as the template. The PCR products were transferred into pEGFP- N2 vector using gene recombinant techniques. The plasmid of interest was detected by restrictive enzyme digestion and PCR, and then transfected into BGC-823 cells by lipid reagent. Results The pEGFP-N2-FAM3B eukaryotic expression vector was constructed successfully. The expression of green fluorescence protein （GFP） was observed in the human gastric cancer cell line BGC-823 after pEGFP-FAM3B transfection under fluorescence microscopy. Conclusion The eukaryotic expression vector pEGFP-N2-FAM3B was successfully constructed and expressed in BGC-823 cells.
作者 李海军 王孟薇 吴本俨 王昌正 王卫华 董雪娟
机构地区 中国人民解放军总医院南楼消化科
出处 《中国药物与临床》 CAS 2007年第12期896-898,共3页 Chinese Remedies & Clinics
基金 军队十五重点基金资助项目(01Z035)
关键词 胃肿瘤 细胞凋亡 真核载体 基因转染 Gastric neoplasms Apoptosis Eukaryotic rector Gene transfection
分类号 R735.2 [医药卫生—肿瘤]
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参考文献12

  • 1Zhu Y, Xu G, Patel A, et al. Cloning, expression, and initial characterization of a novel cytokine-like gene family. Gellomics, 2002,80(2): 144-150.
  • 2Cao X, Gao Z. Robert CE, et al. Pancreatic-terived factor (FAM3B). a novel islet cytokine, induces apoptosis of insulin-secreting beta-cells. Diabetes, 2003,52(9):2296-2303.
  • 3杨少波,王孟薇,邵勇,吴本俨,尤纬缔,李红.应用cDNA微阵列芯片筛选胃癌相关基因[J].肿瘤防治杂志,2004,11(2):117-120. 被引量：9
  • 4Lortz S, Tiedge M, Nachtwey T, et al. Protection of insulin-producing RINm5F cells against cytokine-mediated toxicity through overexpression of antioxidant enzymes. Diabetes, 2000,49:1123-1130.
  • 5Hoorens A, Stange G, Pavlovic D, et al. Distinction between interleukin-l-induced necrosis and apoptosis of islet cells. Diabetes, 2001, 50:551-557.
  • 6Aurora R, Rose GD. Seeking an ancient enzyme in Methanococcus jannaschii using ORF, a program based on predicted secondary structure comparisons. Proc Natl Acad Sci USA,1998,95: 2818-2823.
  • 7Cao X, Yang J, Burkhardt BR, et al. Effects of overexpression of pancreatic derived factor (FAM3B) in isolated mouse islets and insulin-secreting beta TC3 cells. Am J Physiol Endocrinol Metab, 2005,289(4):543-550.
  • 8Yang J, Gao Z, Robert CE, et al. Structure-function studies of PANDER, an islet specific cytokine inducing cell death of insulin-secreting beta cells. Biochemistry, 2005, 44 (34):11342-11352.
  • 9Xu W, Gao Z, Wu J, et al. Interferon-gamma-induced regulation of the pancreatic derived cytokine FAM3B in islets and insulinsecreting beta TC3 cells. Mol Cell Endocrinol,2005,240(1-2):74-81.
  • 10Yang J, Robert CE, Burkhardt BR, et al. Mechanisms of glucoseinduced secretion of pancreatic-derived factor (PANDER or FAM3B) in pancreatic beta-cells. Diabetes, 2005, 54(11):3217-3228.

二级参考文献39

  • 1Wan J, Zhang ZQ, Zhu C, et al. Evaluation of the clinical screening and the follow-up for early gastric cancer by gastroscopy in the elderly. Chin J Geriatr, 2001, 20∶103-104.[万军, 张子其, 朱成, 等. 胃镜普查及随访对老
  • 2Quackenbush J. Microarray data normalization and transformation. Nat Genetics, 2002, 32 (suppl)∶496-501.
  • 3Eisen MB, Spellman PT, Brown PO, et al. Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A, 1998, 95∶14863-14868.
  • 4Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A, 2001, 98∶5116-5121.
  • 5Xiao Y, Segal MR, Rabert D, et al. Assessment of differential gene expression in human peripheral nerve injury. BMC Genomics, 2002, 3∶28.
  • 6Hippo Y, Taniguchi H, Tsutsumi S, et al. Global gene expression analysis of gastric cancer by oligonucleotide microarrays. Cancer Res, 2002, 62, 233-240.
  • 7Sorlie T, Perou CM, Tibshirani R, et al. Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proc Natl Acad Sci U S A, 2001, 98∶10869-10874.
  • 8Moschos SJ, Mantzoros CS. The role of the IGF system in cancer: from basic to clinical studies and clinical applications. Oncology, 2002, 63∶317-332.
  • 9Wu MS, Wang HP, Lin CC, et al. Loss of imprinting and overexpression of IGF2 gene in gastric adenocarcinoma. Cancer Lett, 1997, 120∶9-14.
  • 10Yi HK, Hwang PH, Yang DH, et al. Expression of the insulin-like growth factors (IGFs) and the IGF-binding proteins (IGFBPs) in human gastric cancer cells. Eur J Cancer, 2001, 37∶2257-2263.

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中国药物与临床
2007年 第12期

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