摘要
目的：从基因角度为新型隐球菌性脑膜炎的快速诊断提供一种新方法．方法：应用聚合酶链反应技术检测和鉴定脑脊液中的新型隐球菌特异性基因．结果：对引物的特异性试验证明具有较高的特异；对引物的敏感性试验可扩增出１０个菌细胞；用墨汁复染、分离培养、ＰＣＲ技术，对１５例脑膜炎患者的脑脊液同时进行检测，结果分别为：６６．６％、８６．６％、９３．３％．结论：以新型隐球菌特异性基因片段进行体外扩增，对新型隐球菌性脑膜炎的早期诊断具有重要的临床意义．
Objective: The study was to provide a method for rapid diagnosis of cryptococcal meningitis on the gene level. Methods: PCR method was used to detect specific gene of Cryptococcus neoformans in the cerebrospinal fluid. Results: The primers were proved of high specificity. 10 C. neoformans cells could be detected sensitively. The detection rate of ink counter staining, bacterium culture and PCR was separately 66.6%, 86.6% and 93.3% in the assay of cerebrospinal fluid of 15 cryptococcal meningitic patients. Conclusion: PCR is of momentous clinical singnificance for diagnosing cryptococcal meningitis in the early stage.
出处
《第四军医大学学报》
1997年第4期336-338,共3页
Journal of the Fourth Military Medical University
基金
陕西省科技发展研究计划资助项目
