摘要
目的：建立强力防感合剂质量控削方法。方法：采用薄层色谱法对强力防感合剂中黄芪甲苷和芍药苷进行定性鉴别，用高效液相色谱法，以十八烷基硅烷键合硅胶（4．6mm×200mm，5μm）为固定相。以乙腈-水（13：87，v／v），流速：1．0mL/min；检测波长：230min，对强力防感合剂中芍药苷进行含量测定。结果：薄层色谱鉴别方法专属性强。芍药苷检测量在0．16—0．8μg与峰面积积分值呈良好的线性关系（r=0．9998），平均回收率为100．23％，RSD=1．58％（n=6），芍药苷含量以不少于200μg/mL为质控标准。结论：该方法准确、简便、易行，可用于强力防感合剂质量控制。
Objective：To establish the quality control method for Qiangli Fanggan mixture. Me,otis： TLC was used in qualitative discrimination of astragaloside and paeoniflorin. The paeoniflorin assaying was conducted with the external standard method with a column of C18 （4.6 mm × 200 mm, 5 μm）, mobile phase of acetonitrile-water （13：87, v/v） and flow rate of 1.0 mL/min at the wavelength of 230 nm. Results： TLC had a strong specificity, and a stable praeparatum character. The peak area of paeoniflorin showed a good linearity in the range of 0.16 - 0.8 μg （ r = 0.9998）. The average recovery was 100.23%, RSD = 1.58% （ n = 6）. The standard of qualified Qiangli Fanggan mixture was no less than 200 μg/mL of praeparatum. Conclusions： The method is simple, accurate and suitable, and can be used as the quality control for Qiangli Fanggan mixture.
出处
《儿科药学杂志》
CAS
2007年第6期33-35,共3页
Journal of Pediatric Pharmacy
