摘要
目的研究新试剂2-[2-＇（5-硝基-吡啶）-偶氮]-变色酸（5-NO2-PACA）的合成方法。方法以5-硝基-2-氨基吡啶为原料,经重氮化,再与变色酸偶联合成新试剂,利用试剂与钴在pH=10.00的缓冲介质中的显色反应进行钴（Ⅱ）含量测定。结果得到紫红色晶体,并用元素分析、红外光谱和氢核磁共振谱鉴定了其结构;试剂与钴形成1∶1稳定绿色配合物,λmax=680 nm,ε=8.07×10^4L/mol·cm,钴的测定线性范围为0-10μg/25 mL。结论该法简单易行,准确可靠,可以用于VB12针剂中钴（Ⅱ）含量的测定。
Aim To synthesize 2-[ 2＇-（5-nitro-pyridylazo） ] -Chromotropic acid （ 5-NO2-PACA） and develop the newmethod of determination of cobalt（ Ⅱ ）. Methods Using 5-nitro-2-nitylpyridine as raw material, and by diazoiation, then was coupled with chromotropic acid to synthesize new ingrain of 2- [ 2＇- （5-nitro-pyridylazo） ] -chromotropic acid. The method of determination of cobalt was developed based on the reageng with Co （ Ⅱ ） to color reaction in the pH = 10. 00. Results The amaranth complex was produced and the structure of the reageng was char- acterized by elemental analysis and infrared absorption spectrometry. The reageng with Co （ Ⅱ ） to form a stable 1： 1 green complex. The apparent molar absorptivity was 8.07×10^4L/mol·cm at λmax, = 680 nm. The ranges of determination are 0 - 10 μg/25 mL for Co（ Ⅱ ）. Conclusion The method has been applied satisfactorily to the determination of Co（ Ⅱ ） in the injection of VB12.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第5期763-766,共4页
Journal of Northwest University（Natural Science Edition）
基金
陕西省自然科学基金资助项目(N02002D21)
陕西省教育厅基金资助项目(SJG0503)
