摘要
目的研究低氧诱导因子(HIF-1α)和核因子(NF-κB)在小鼠视网膜光损伤后的表达及低氧预适应对其表达的影响,探讨低氧预适应对视网膜光损伤的保护机制。方法将54只BALB/C小鼠随机分成单纯光照组、低氧预适应组和正常组,前2组又按光照后处死时间的不同分为6、12、36h和7d组。应用免疫组织化学技术检测小鼠视网膜组织HIF-1α和NF-κB蛋白的表达,并分析低氧预适应组与单纯光照组HIF-1α和NF-κB蛋白的表达差异。结果正常小鼠视网膜组织HIF-1α几乎不表达,NF-κB有微量表达。低氧预适应组与单纯光照组比较,HIF-1α的表达强度明显增强,而NF-κB的表达强度明显减弱,两组比较差异有统计学意义(P<0.01)。结论低氧预适应可能通过提高HIF-1α的表达有效抑制NF-κB信号通路,从而通过降低凋亡的发生而起到保护作用。
Objective Hypoxic preconditioning can activate endogenous neuroprotective system to protect retinal light injury and has a bright future in the treatment of retinal disease. Present paper was to study the expression of hypoxic induced factor-1α （HIF-1α） and nuclear factor-κB（NF-κB） in the retina of light damage mouse and investigate the possibly protective pathogenesis of hypoxic preconditioning against it. Methods Fifty-four BALB/C mice were randomly divided into simple light exposure group, hypoxic preconditioning group and normal control group. The former two groups were subdivided into light exposure 6,12,36 hours groups and 7 days group. Expressions of HIF-1α and NF-κB were detected by immunohistochemical staining. Results No HIF-la,NF-κB positive cells were found in normal control group. In simple light exposure group,a few of NF-κB were expressed in 6 hours. The expressions of HIF-1α and NF-κB were gradually increased with time and reached peak in 12 hours,and then decreased in 36 hours and 7 days. Expression of HIF-1α followed the same fashion as NF-κB. The same trend in expressions of HIF-1α and NF-κB also was noted in hypoxic preconditioning group, but showed an evidently weak expressive intensity in NF-κB compared with HIF-1α in each time point with a statistically significant difference（ P 〈 0.01 ）. Conclusion Hypoxic preconditioning increases the expression of HIF-1α by inhibiting NF-κB signaling cascades,and it plays the protection through suppressing the apoptosis of retinal cells.
出处
《眼科研究》
CSCD
北大核心
2007年第12期941-944,共4页
Chinese Ophthalmic Research
