摘要
目的:从三列凹顶藻Laurenciatristicha中寻找具有多样性结构的化学成分,供药理活性筛选。方法:采用凝胶柱色谱、硅胶柱色谱、重结晶和高效液相色谱等方法进行分离;借助核磁共振等波谱方法鉴定化合物的结构;用MTT法对得到的化合物进行细胞毒活性评价。结果:分离得到7个化合物,分别鉴定为鉴定为胆甾醇(1),胆甾-5-烯-3β,7βα二醇(2),β-谷甾醇(3),叶绿醇(4),玉米黄素(5),对羟基苯甲醛(6),3-吲哚甲醛(7);在人肿瘤细胞株HCT-8,Bel-7402,BGc-823,A549和HELA模型上,化合物2对所有细胞株均显示毒性,化合物4对HCT-8和HELA细胞显示中等强度的细胞毒活性,其它化合物对所有细胞株均无明显毒性,IC50均大于10.0μ·mL^-1。结论:化合物1—7均为首次从三列凹顶藻中得到,化合物2对所有细胞株均显示毒性,化合物4对HCT-8和HELA细胞具有中等强度的选择性细胞毒活性。
Objective: To search for chemical constituents with structural diversity from Laurencia tristicha to supply for biological assay. Method: Compounds were isolated by means of column chromatography over normal phase silica gel and Sephadex LH - 20, recrystallization and HPLC. Structures were identified by spectroscopic methods including 1D NMR, IR and MS. Cytotoxicities of the purified compounds were evaluated by MTF method. Result: Seven compounds were isolated from L. tristicha. Their structures were elucidated as cholesterol (1), cholesta- 5-en-3β, 7α-diol (2), β-stigmasterol (3), phytol (4), zeaxanthin (5), 4 -hydroxy- benzaldehyde (6), indolyl-3-carbaldehyde (7). In the cytotoxic assay compound 2 was active against human cancer cell lines HCT-8, Bel-7402, BGc-823, A549 and HELA with IC50 values of 1.90, 2.02, 1.99, 6.52 and 1.20 μg · mL^-1 , respectively. Compound 4 showed cytotoxicity against HCT-8 and HELA with ICso value of 3.51 and 2. 04 μg · mL^-1 , and other compounds were inactive (IC50 〉 10 μg · mL^-1 ). Conclusion: Compounds 1-7 were isolated from L. tristicha for the first time. In additon, compounds 2 and 4 were cytotoxic against several human cancer cell lines.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第24期2610-2612,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金(20432030)
鲁东大学校博士基金(LY20063302)
关键词
三列凹顶藻
化学成分
细胞毒活性
Laurencia tristicha
chemical constituents
cytotoxic activity