摘要
目的:建立快速鉴定食源性金黄色葡萄球菌的基因芯片技术。方法:采用PCR方法扩增金黄色葡萄球菌16SrRNA基因的DNA片段,序列进行BLAST比较并通过软件设计其特异性探针,采用基因芯片杂交技术鉴定食源性金黄色葡萄球菌样品。结果:对所有样品进行基因芯片杂交技术处理并扫描观察,金黄色葡萄球菌的杂交结果呈阳性,其检测结果与传统方法鉴定结果一致。结论:应用基因芯片鉴定技术可快速、准确地鉴定食源性金黄色葡萄球菌,值得推广应用。
Objective： The goal of this study was to establish a method for identifying rapidly food-borne Staphylococcus aureus by microarray technology. Methods： The target gene of 16SrRNA form S.aureus was amplified by PCR （polymerase chain reaction） and the sequence was compared for BLAST（basic local alignment search tool） in genbank. The oligonucleotide hybridization probe was designed to identify samples. Results： The specimens were detected by microarray hybridization. The result of microarray hybridisation for food-borne Staphylococcus aureus showed that it is positive. Conclusion： The foodborne Staphylococcus aureus is identified rapidly and accurately with microarray hybridization technique so it is worth propagation.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第12期294-297,共4页
Food Science
基金
四川省应用基础研究项目(04JY029-053-1)
西华大学人才引进项目
关键词
金黄色葡萄球菌
基因芯片
鉴定
Staphylococcus aureus
microarray： identification
