摘要
为探讨pH值和甘油对藏獒精液冷冻保存前后精子活力的影响，筛选6条种公藏獒在其繁殖季节（9—12月份）以不同pH值梯度（5．5，6．0，6．5，7．0，7．5）稀释液和不同甘油平衡时间（10，20，30，40，50，60，70，80，90min）对藏獒精液进行冷冻，检查精子活力变化。结果表明，在藏獒精液冷冻过程中，pH值为6．5的稀释液中精子在冷冻前后有较高的活力；甘油平衡时间对藏獒精液活力有较大影响，冻前精子活力随甘油作用时间而下降，在甘油平衡时间为20min所得藏獒冻后精子活力效果较好。
A The effect of different extender pH （5.5,6.0,6.5,7.0,7.5） and different balance time （10, 20,30,40,50,60,70,80,90 min）of Glycerin on the motility of frozen sperm of Tibetan Mastiff in their reproduction season （Sep- Dec） was studied in the experiment. The results showed that the motility of sperm was higher in ph 6.5 extender than in the others in the condition of 4 ℃. The balance time of glycerin affected the motility of sperm greatly, and the balance time of 20 min was better for motility of frozen sperm than that of others.
出处
《经济动物学报》
CAS
2007年第4期202-205,共4页
Journal of Economic Animal
关键词
藏獒
精子
活力
PH值
平衡时间
Tibetan Mastiff
semen
motility
pH
time of balance
