摘要
目的探索移植转染胶质细胞源性神经营养因子（GDNF）基因的大鼠神经干细胞（NSCs）能否促进脑损伤大鼠的神经功能修复。方法利用阳离子脂质体转染GDNF基因到大鼠NSCs，在脑立体定向仪引导下分别将PBS、NSCs、转基因NSCs移植到脑损伤大鼠局部损伤灶边缘，通过观察记录大鼠行为能力的变化，评价移植细胞后大鼠神经功能的修复情况。结果转染后72h，荧光蛋白大量表达。转基因细胞移植后可以在14d时仍表达GDNF基因，各实验组于移植后3d时行为学指标差异无统计学意义（P〉0．05），而在移植后7d，移植转基因NSCs组和NSCs组即与对照组在行为学指标上差异有统计学意义（P〈0．05）；在移植后14d，移植转基因NSCs组与其余两组在行为学指标上差异均有统计学意义（P〈0．05）。结论转基因NSCs移植后可以分泌GDNF并促进脑外伤大鼠神经功能的恢复。
Objective To explore if the transplantation of neural stem cells （NSCs） transfected with glial cell line-derived neurotrophic factor （GDNF） could promote the nerve functional recovery in rats with traumatic brain injury. Methods Gene GDNF was transfected into neural stem cells with li- pofectamine. PBS, NSCs and gene-transfected NSCs were transplanted into the surrounding focus of brain injury in rats respectively following traumatic brain injury （Control group, Neural stem cell group and Gene-transfected group） guided by cerebullar stereotaxic apparatus. The nerve functional recovery after transplantation in each group was evaluated. Results Fluorescence proteins were mostly expressed at 72 h following transfection and there was also expression of gene GDNF at day 14 in Gene-transfected group. No significant difference was found in the behavior indicators among the groups at day 3 after transplantation （ P 〉 0.05 ）, while the behavior indicators in Gene-transfected group were significantly different from those of Control group at day 7 （P 〈0.05） and from those of the other two groups at day 14 （ P 〈 0.05）. Conclusion Transplantation of the gene-transfected NSCs can repair the nerve function of the rats with traumatic brain injury by secreting GDNF.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2007年第12期894-897,共4页
Chinese Journal of Trauma
