期刊文献+

链霉菌702原生质体的制备和再生条件研究

Studies on Condition of Formation and Regeneration of Protoplasts from Streptomyces 702
下载PDF
导出
摘要 为了确定链霉菌702菌株原生质体制备和再生较优组合条件,以链霉菌702菌株为试验材料,试验设计以单因素和多因素多水平的正交试验。在单因素试验结果中探索该菌的对数生长期为35 h^50 h,在菌丝体培养基中添加1.0%甘氨酸有利于该菌原生质体制备和再生;正交试验分别以影响该菌原生质体制备和再生的菌丝体培养时间、溶菌酶使用浓度、酶解温度和酶解时间的四因素三水平的L9(34)正交试验,试验表明:链霉菌702菌株原生质制备和再生较优组合为A2B2C3D1,即菌丝体培养时间为43 h,溶菌酶浓度为2.0 mg/mL,酶解温度为37℃,酶解时间60 m in,原生质体的制备率和再生率分别达到96.5%和27.8%。本试验为该菌进一步进行原生质体诱变打下良好的基础。 The better composition condition of the factors that affected the formation and regeneration of protoplasts from Streptomyces 702 were investigated. The experimental material was Streptomyces 702. And single factor and multifactor orthogonal experiment were used in this experiment. From single factor experiment the result proved that the logarithmic phase of this Streptomyces was 35 h - 50 h. Furthermore, it was propitious to form and regenerate the protoplasts of Streptomyces 702 that 1.0% of glycin was added in mycelium substrate. The L9 (3^4) orthogonal experiment was mycelium cultured time, lysozyme concentration, enzymolysis temperature and time. By using range analysis, varimace analysis and aggregative score, we analyse the outcome of the test. The results indicated that the better assembly condition of formation and regeneration of protoplasts from Streptomyces 702 was A2B2C3D1 ( mycelium cultured for 43 h, 2 mg/mL of lysozyme concentration, enzymolysis at 37℃ for 60 min). The protoplasts rate of forming and regenerating was 96.5% and 27.8%. It laid good foundation for further mutagenesis of the protoplasts.
出处 《江西科学》 2007年第6期733-736,共4页 Jiangxi Science
基金 江西省自然科学基金(No.050010) 广东省科技攻关项目(No.2006B13001004)。
关键词 链霉菌702 原生质体 制备与再生 综合评分法 Streptomyces 702, Protoplasts, Formation and regeneration, Aggregative score
  • 相关文献

参考文献6

二级参考文献42

  • 1彭帮柱,岳田利,袁亚宏,王丽威.酵母菌原生质体融合技术[J].西北农业学报,2004,13(1):101-103. 被引量:24
  • 2孟平蕊,王永红,杨春霞,霍庆来.正交实验法微机化及其在有机合成实验教学中的应用[J].山东建材学院学报,1994,8(3):94-97. 被引量:5
  • 3霍普伍德.链霉菌遗传操作实验手册[M].长沙:湖南科学技术出版社,1988.224-225.
  • 4Iain S Hunter.链霉菌的基因克隆化[J].国外医药:抗生素分册,1988,9(4):241-251.
  • 5[1]R.V.Muralidhar,T.Panda.Fungal protoplast fusion.Bioprocess Engineering,2000,22:429-431
  • 6[6]Shang-Shyng Yang,Chiou-Mei Lei.Formation and regeneration of protoplast for protease production in Streptomyces rimosus.Microbiol Immunol Infect,2001,34:8-16.
  • 7[7]Yali Cheng,Richard R.Belanger.Protoplast preparation and regeneration from spore of biocontrol fungus Pseudozyma flucculosa.FEMS Microbiology Letters,2000,190:287-291.
  • 8[8]N.Balasubramanian,G..Annie Juliet,P.Srikalaivani,D.Lalithkumari.Release and regeneration of protoplast from the fungus Trichothecium roseum.Can.J.Microbiol,2003 (49):263-268.
  • 9[11]Manisha V.Chitnis,Mukund V.Deshpande.Isolation and regeneration of protoplasts from the yeastand mycelial form of the dimorphic zygomycete Benjaminiella poitrasii:Role of chitin metabolismfor morphogenesis during regeneration.Microbiol.Res.2002,157,29-37
  • 10[12]Sanae Kano,1 Tadanori Aimi,2 Seita Masumoto,1 Yutaka Kitamoto.Physiology and Molecular Characteristics of a Pine Wilt Nematode Trapping Fungus,Monacrosporium megalosporum.Current Microbiology,2004,49:158-164

共引文献125

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部