摘要
采用毛细管电泳–鲁米诺电致化学发光技术建立了检测儿茶素的新方法。考察了检测池溶液和运行缓冲液pH值、鲁米诺浓度、进样电压和进样时间、检测电位等测试条件对分离及发光强度的影响。在优化的条件下,儿茶素浓度在8.0×10–6～1.0×10–3mol/l范围内与发光强度呈良好的线性关系,相关系数大于0.99,检出限为1.0×10–6mol/l。对1.0×10–3mol/l儿茶素平行测定5次,出峰时间和出峰强度的相对标准偏差(RSD)分别为0.64%和4.1%。用该方法对中药材儿茶中儿茶素进行了测定。
A method of capillary electrophoresis （CE）withLuminol-electrogenerated chemiluminescence （ECL） detection has been developed for the determination of catechin. Separation column was a 25 μm i.d. capillary. The running buffer was 50 mmol/l boric acid ＋ 25 mmol/l potassium dihydrogen-phosphate buffer （pH=8.0）. The solution in the detection cell was 1,0 mmol/l Luminol ＋ 0.3 mol/l KC1 （pH 10.5）. Other optimized conditions comprise electrokinetic injection at 16 kV for 10 s, seperation voltage at 12 kV and detection potential at 0.6 V. A linear relation between ECL intensity and catechin concentration was obtained in the range of 8.0xl0^-6l.0xl0^-3mol/l, with a detection limit of 1.0xl0^-6mol/l. The relative standard deviations （n=5） of migration time and ECL intensity were 0.64% and 4.1%, respectively, for the detection of 1.0xl0^-3mol/l catechin. The method was successfully applied to the determination of catechin in Chinese herb catechu with satisfactory result.
出处
《传感器世界》
2007年第12期11-14,共4页
Sensor World
基金
国家自然科学基金资助项目(编号:20776033)
关键词
毛细管电泳
电致化学发光
鲁米诺
儿茶素
capillary electrophoresis
electrogeneratedchemiluminescence
Luminol
（＋）-catechin
