摘要
以蓝刺头(Echinops latifoliusTausch.)叶片为外植体,建立了蓝刺头的再生体系,并对其影响因素进行了研究。结果表明,MS+6-BA 1.0mg.L-1+NAA 0.1mg.L-1为最适分化培养基,再生率达48.33%,平均每个外植体再生芽数3.56;添加2.0mg.L-1的硝酸银以及暗处理15d均可显著提高不定芽的再生率和再生芽数,再生率分别提高到80.33%和63.33%。
Using leaves as explants, we established a high frequency regeneration system of Echinops latifolius Tausch, and studied the main factors which affecting plant regeneration in vitro. The results showed that the optimum medium for shoot regeneration was MS ＋ 6-BA 1.0mg·L^-1 ＋ NAA 0. 1mg·L^-1. The frequency of shoot regeneration was 48.33 % and mean number of buds per explant was 3.56. The optimum medium plus 2.0mg·L^-1 AgNO3, and incubating for 15 days under dark condition could strikingly promote shoot regeneration and the number of bud, with frequency of 80.33 % and 63.33 % respectively.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第6期581-584,共4页
Journal of Nuclear Agricultural Sciences
基金
北京市林业局花卉产业处资助项目(25012482)
关键词
蓝刺头
再生体系
硝酸银
暗处理
Echinops latifolius Tausch.
regeneration system
AgNO3
dark incubation
