摘要
目的:探讨复方中药对间接原位移植肝癌裸鼠模型的干预作用。方法:采用人肝细胞癌细胞株Bel-7402间接原位移植,造雄性肝癌裸鼠模型48只,造模后24 h随机分4组,即复方中药低(A)、中(B)、高(C)3个浓度组和FT207化疗组(D),4周后处死裸鼠,观察各组荷瘤裸鼠的生存时间、瘤体积、瘤指数、肝内转移情况等,并用二步法免疫组化检测肝组织、肝癌旁组织、肝癌组织中PTEN蛋白的表达。结果:四周时,中药组(A+B+C)总存活率为50.0%,高于化疗组0%(P<0.01);A组存活率虽高于D组,但无显著性意义(P>0.05);B组和C组生存率均明显高于D组(P<0.05或P<0.01)。中药组的瘤体积和瘤指数与化疗组相比无明显差异(P>0.05)。经中药治疗的癌鼠肝内转移少于化疗组癌鼠(P<0.05)。瘤体积与模型生存时间负相关,与脾指数正相关(P<0.01)。PTEN蛋白表达强度以癌旁组织最高,癌组织最低,正常肝组织次之(P<0.01)。中药治疗相对于FT207化疗而言可明显增加PTEN蛋白的表达强度,中药组组间比较提示中浓度组效果最好(P<0.05或P<0.01),低浓度和高浓度差别不大(P>0.05)。结论:本方能延长实验癌鼠的生存时间,减少癌灶的肝内转移,肿瘤体积过大是瘤鼠的主要死因,其治疗肝癌的部分机制可能是通过增强PTEN蛋白表达来实现的。
Objective： To research the treatment effect of a complex prescription of Chinese crude drug in BALB/c athymic mice Methods： 48 male BALB/c athymic mouse models were built by Bel-7402 with an indirect method.After 24 hours of postoperation,they were distributed randomly into 4 groups which were treated by intragastric administration with complex prescription of Chinese crude drug of different density as the low,middle,high and FT207（Tegafur） for 4 weeks.At last, athymic mice were put to death and survial time,volume of tumors,exponent of tumors and the tumor metastasis in livers were observed;and PTEN was detected in hepatic tissue,latero-cancer tissue and cancer tissue by immunohistochemistry.Results： 4 weeks later,the total survival rate in treatment group（A＋B＋C）was 50% and more higher than the control group（0%） treated by FT207,P〈0.01.The survival rate in group A,B,C was higher than group D,except group A,there was significant differences,P〈0.05 or 0.01.And no differences were observed between the treatment groups and the control group in volume of tumors and exponent of tumors,P〉0.05.Tumor metastasis in livers of the treatment group is less than the control＇s,P〈0.05.Negative correlation between volume of tumors and survival time,positive correlation between tumors volume and spleen exponent were observed,P〈0.01.The result of immunohistochemistry represented that the intensity of PTEN in latero-cancer tissue was the highest,and then the hepatic tissue,the lowest was cancer tissue,P〈0.01.It also represented that the intensity of PTEN in treatment groups（A,B,C）was more higher than the control group（D）,and group B was the highest in the treatment groups,P〈0.05 or P〈0.01.However,there was no significant statistic difference between group A and group C,P〉0.05.Conclusion： This complex prescription of Chinese crude drug can prolong the survival time and decrease tumor metastasis in livers of these experimental mice.Too large tumor volume is an main death cause of model.Mechanisms of this complex prescription of Chinese crude drug healing HCC may partially be explained by enhancing the expression of PTEN in liver.
出处
《中药材》
CAS
CSCD
北大核心
2007年第11期1417-1421,共5页
Journal of Chinese Medicinal Materials
基金
广东省科技计划项目(2005B33001040)
广东省中医药局资助项目(1040056和301014)
关键词
复方中药
裸鼠肝癌模型
生存时间
肝内转移
PTEN
Complex prescription of chinese crude drug
HCC-model of athymic mouse
Survial time
Tumor metastasis in livers
PTEN
