摘要
目的：建立以反相高效液相色谱法测定天母降压片中天麻素含量的方法。方法：色谱柱为Shimadzu-C18（150mm×4．6mm，5μm），流动相为乙腈-水（0．5：99．5），流速为1．0mL·min^-1柱温为40℃，检测波长为221nm。结果：天麻素进样量在0.2712～1．3560μg范围内与峰面积积分值呈良好的线性关系（r=0.9999）；平均加样回收率为100．13％，RSD=1．89％（n=6）。结论：本法简便、准确、专属性强、回收率高，可用于天母降压片的质量控制。
OBJECTIVE ： To establish an RP - HPLC method for the quantitative determination of Gastrodin in Tianmujiangya tablets.METHODS： The chromatographic separation was performed on Shimadzu- C18（150mm × 4.6mm, 5μm） column with a column temperature at 40℃ , The mobile phase consisted of acetonitrile - water（0.5 ： 99.5） at a flow rate of 1.0mL · min ^-1. The detection wavelength was set at 221nm. RESULTS： The linear correlation of Gastrodin was achieved in the range of 0.271 2μg-356 0μg（r = 0.999 9） . The average recovery rate was 100.13% （RSD - 1.89%, n = 6） . CONCLUSION： The method is simple, accurate and specific and with high recovery rate, and it can be used for the quality control of Tianmujiangya tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第36期2843-2844,共2页
China Pharmacy
