摘要
从浙江省临安、桐庐等地桑园中的"凋萎"桑树根、茎部获得151个细菌分离物样品。经离体水培接种试验表明,这些分离物均可引起桑树"凋萎"症状,其形态、大小、染色反应、培养性状与对照番茄青枯菌(Ralstonia so-lanacearum)相近;PCR鉴定其中125个分离物为雷尔氏菌(Ralstonia solanacearum)。结合田间发生症状,将桑树"凋萎"症状诊断为细菌性青枯病。从125个雷尔氏菌株中选择16个典型菌株进行研究,根据生理小种划分标准,这些菌株均为雷尔氏菌的生理小种1,其中75%为青枯菌的生化型Ⅴ、25%为青枯菌的生化型Ⅲ。
One hundred and fifty-one bacterial strains were isolated from the roots and stems of wizened mulberry in Ling＇an and Tonglu counties of Zhejiang Province. Pathogenicity test results show that all of the strains can induce the mulberry wilt, and the shape, size, dye-reaction and culture characteristics of the mulberry wilt were similar to those of the tomato bacterial wilt. Based on PCR results, 125 bacterial strains were identified as Ralstonia solanacearum, Based on the symptom in farm, the mulberry wilt were diagnosed as bacterial wilt. Among them, 16 typical strains were also classified as the physiological race 1 by Haywind＇s classification criterion, and 75% of which were Ralstonia solanacearum biovar Ⅴ, 25% were Ralstonia solanacearum biovar Ⅲ.
出处
《蚕业科学》
CAS
CSCD
北大核心
2007年第4期528-533,共6页
ACTA SERICOLOGICA SINICA
基金
浙江省重大科技攻关项目(编号2006C12057)
关键词
桑树
凋萎症状
细菌性青枯病
雷尔氏菌
Mulberry
Wilt character
Bacterial wilt
Ralstonia solanacearum
