摘要
克隆了家蚕苏氨酸脱氢酶基因(Bmtdh),其cDNA长1 310 bp(No.ABA43639.1),包括227 bp 5′-UTR和1 026 bp的完整开放读码框(ORF)。Bmtdh在家蚕基因组上以单拷贝形式存在,编码342个氨基酸,其Ile29-Val322区域是一个完整的表面异构酶功能域。表达谱分析与EST数据的分析表明,Bmtdh在丝腺和卵巢中的转录水平高于其它组织,并且Bmtdh在家蚕4龄眠期、5龄早期的转录水平也很高,而在卵期(G2胚胎)和1龄早期没有检测到其转录表达,表明Bmtdh基因可能参与了蚕丝蛋白的合成过程。
We cloned and sequenced the cDNA （1 310 bp, accession No. ABA43639. 1） of L-threonine 3-dehydrogenase （TDH） in Bombyx mori. It contains a 227 bp 5＇ untranslated region（ 5＇-UTR ）, locates on the ge nome as a single copy and encodes a 342-residue protein. The region between Ile29 and Val322 is a complete episomerase domain. The expression profile and EST analysis indicated the mRNA transcription level of Bmtdh gene was higher in silk gland and ovary at the 3^rd day of 5^th instar than that of other tissues. RT-PCR experiments of 11 different development periods showed that high transcription level of Bmtdh gene appeared at the 4^th molting stage, 1^st day and 3^rd day of 5^th instar, but it was not detected in G2 embryo period and at the 2^nd day of 1^st instar. This results suggested the Bmtdh gene maybe involved in the process of silk protein synthesis.
出处
《蚕业科学》
CAS
CSCD
北大核心
2007年第4期553-561,共9页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(编号30400328)
国家重点基础研究发展计划"973"项目(编号2004AA2Z1020)
重庆市自然科学基金项目(编号9067)
关键词
家蚕
苏氨酸脱氢酶基因
丝腺
克隆
序列分析
Bombyx mori
L-threonine 3-dehydrogenase gene
Silk gland
Clone
Sequence analysis
