摘要
目的探讨负载CTLA4Ig重组腺病毒的未成熟树突状细胞对大鼠Th细胞增殖的影响。方法将CTLA4Ig重组腺病毒与Wistar大鼠未成熟树突状细胞于37℃共孵育6h后，经尾静脉注射该大鼠作实验组，另外分别设立Wistar大鼠未成熟树突状细胞、CTLA4Ig重组腺病毒、生理盐水经尾静脉注射为对照。1周后，用0．3％戊巴比妥麻醉各组大鼠后抽血检测CTLA4Ig。取脾脏，经流式细胞术分选出Th1、Th2细胞及CD4^＋ T细胞，行混合淋巴细胞培养检测Th细胞的增殖。用免疫组织化学法检测Th1、Th2细胞的比例。结果实验组血清CTLA4Ig水平（0．654±0．13）显著高于CTLA4Ig重组腺病毒组（0．392±0.10，P〈0．01），树突状细胞组及生理盐水组未检出。实验组Th1细胞的增殖指数（742±161）、Th1／Th2（0．16±0．05）均显著低于各对照组（分别与未成熟树突状细胞组、CTLA4Ig重组腺病毒组、生理盐水组相比，P均〈0．01）；而Th2细胞的增殖指数（9162±598）显著高于各对照组（P均〈0．01）。结论负载CTLA4Ig重组腺病毒的未成熟树突状细胞可显著抑制大鼠Th1细胞增殖，促进Th2细胞增殖，使Th细胞由Th1向Th2显著偏移，诱导有效的免疫耐受。
Objective To explore the influence of immature dendritic cells （DCs） loading recombinant adenoviral CTLA4Ig on Th cell proliferation. Methods Recombinant adenoviral CTLA4Ig and immature DCs of Wistar rats were co-incubated in thirty-seven centigrade for six hours. These immature DCs loading recombinant adenoviral CTLA4Ig were injected into Wistar rats by vena caudalis as experimental group. Immature DCs of Wistar rats, recombinant adenoviral CTLA4Ig and normal saline were used as control groups by above injection respectively. One week later, serum CTLAIg was detected and spleen of rat was taken. Thl cells, Th2 cells and CD4^＋ T cells were sorted by flow cytometry. Th cell proliferation was detected by mixed lymphocyte culture. The proportion of Thl cells and Th2 cells was detected by immunohistochemistry. Results Serum CTLA4Ig in experimental group （ 0. 654 ± 0. 13 ） was remarkably higher than those in recombinant adenoviral CTLA4Ig group （ 0. 392 ± 0. 10, P 〈 0.01 ）. No CTLA4Ig was detected in DCs group and saline group. Thl cell proliferation index （742 ± 161 ） and ratio （0.16±0.05 ）of Thl were markedly inferior to those in control groups（ all P 〈 0.01 ） , while Th2 cell proliferation index （9162 ± 598）was significantly higher as compared to controt groups （all P 〈 0.01 ）. Conclusions Immature DCs loading recombinant adenoviral CTLA4Ig can inhibit Thl cell proliferation of rats while facilitate Th2 cell proliferation, which makes Th cells excurse from Thl to Th2 significantly and induces effective immunotolerance.
出处
《现代临床医学生物工程学杂志》
2007年第2期69-73,共5页
Journal of Modern Clinical Medical Bioengineering
基金
广东省自然科学基金（04300824）
