KISS-1及MMP-9在子宫内膜异位症患者异位及在位内膜的表达及意义被引量：6

Expression and significance of KISS-1 and matrix metalloproteinase-9 in ectopic and eutopic endometrium in patients with endometriosis

下载PDF

导出

摘要目的:探讨KISS-1及MMP-9在子宫内膜异位症患者在位及异位内膜组织的表达及意义。方法:用免疫组化方法检测46例患者异位子宫内膜组织及其中16例在位内膜组织和33例正常子宫内膜组织中KISS-1及MMP-9的表达情况。结果:KISS-1在子宫内膜异位症患者异位/在位内膜及正常子宫内膜组织中阳性表达率分别为30.43%、31.25%和63.64%,差异有统计学意义(P<0.05);MMP-9表达率分别为60.87%、56.25%和27.27%,差异有统计学意义(P<0.05);进一步比较KISS-1及MMP-9表达的阳性强度,3组间差异亦有统计学意义(P<0.01)。结论:KISS-1在子宫内膜异位症的表达降低,MMP-9的表达增高可能与内异症的侵袭有关。 Objective ：To study the expression and significance of KISS-1 and MMP-9 in ectopic and eutopic endometrium in patients with endometriosis. Methods：The expressions of KISS-1 and MMP-9 in 33 cases of normal endometrium,46 cases of ectopic endometrium and 16 cases of eutopic endometrium from former 46 endometriosis patients were detected by immunohistochemical method. Results： In ectopic and eutopic endometrium of endometriosis and normal endometrium, the positive expression rates of KISS-1 were 30.43 % ,31.25% and 63.64% respectively. The positive expression rates of KISS-1 in ectopic and eutopic endometriotic tissues were significantly lower than those in the normal endometrium（ P 〈0.05）. The positive rates of MMP-9 in the ectopic and eutopic endometrium of endometriosis and normal endometrium were 60.87% ,56.25% and 27.27% respectively. The positive expression rates of MMP-9 in ectopic and eutopic endometriotic tissues were significantly higher than those in the normal endometrium（ P 〈 0.05 ）. The positive intensity of staining of KISS-1 and MMP-9 were statistically different among these three groups（ P 〈0.01 ）. Conclusion：The decreased expression of KISS-1 and the increased expression of MMP-9 protein may be involved in the invasion of endometriosis.

作者申庆文凌斌席玉玲承泽农杜丹丽

机构地区蚌埠医学院附属医院妇产科安徽省立医院妇产科蚌埠医学院病理科

出处《现代妇产科进展》 CSCD 北大核心 2007年第11期843-845,883,共4页 Progress in Obstetrics and Gynecology

关键词子宫内膜异住症基因 KISS-1 基质金属蛋白酶9 Endometriosis Gene, KISS-1 Matrix metalloproteinases-9

分类号 R737.31 [医药卫生—肿瘤]

引文网络
相关文献

参考文献9

1Szamatowicz J, Laudanski P,Tomaszewska I, et al. Expression of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 : a possible role in the pathogenesis of endometriosis [J]. Hum Reprod, 2002,17 : 284- 288
2Lee JH, Welch DR. Identification of highly expressed genes in metastusis-suppressed chromosome 6/human malignant melanoma hybrid cells using subtractive hybridization and differential display [ J ] Int J Cancer, 1997,71 : 1035-1044
3Yan C,Wang H,Boyd DD. KISS-1 represses 92-kDa type IV collagenase expression by down-regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha-induced block of p65/p50 nuclear translocation [J]. J Biol Chem,2001,276 : 1164-1172
4Tan XJ,Lang JH,Liu DY,et al. Expression of vascular endothelial growth factor and thrombospondin-1 mRNA in patients with endometriosis [ J ]. Fertil Steril, 2002, 27 : 148-153
5Nash KT, Welch DR. The KISS1 metastasis suppressor: mechanistic insights and clinical utility[J]. Front Biosci, 2006,11:647-659
6Goldberg SF, Miele ME, Hatta N, et al. Melanoma metastasis suppression by chromosome 6: evidence for a pathway regulated by CRSP3 and TXNIP [ J ]. Cancer Res, 2003,63:432-440
7牛慧彦,王丹波,张淑兰,甘淑君.KISS-1和MMP-9在裸鼠子宫内膜异位症模型中的表达及意义[J].中国医科大学学报,2004,33(6):487-489. 被引量：20
8Ramom L, Gilabert-Estelles J, Castello R, et al. mRNA analysis of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis using a real-time quantitative RT-PCR assay [ J ]. Hum Reprod, 2005,20 : 272-278
9李艳,郎景和,王立杰,许秀英.MMP-9及TIMP-1在子宫内膜异位症患者腹水及血清中的表达[J].现代妇产科进展,2005,14(4):293-296. 被引量：9

二级参考文献18

1Lee JH, Miele ME, Hicks DJ, et al. KISS-1, a novel human malignant melanoma metastasis - suppressor gene [J]. J Natl Cancer Inst, 1996, 88(23) :1731 - 1737.
2Osteen KG, Bruner KL, Sharpe-Tinms KL. Steroid and growth factor regulation of matrix metalloproteinase expression and endometriosis [J]. Semin Reprod Endocrinol, 1996, 14(3): 247 -255.
3Awwad JT, Sayegh RA, Tao XJ, et al. The SCID nude mouse: an experimental model for endometriosis [J]. Hum Reprod, 1999, 14(12): 3107-3111.
4Nisolle M, Casanas-Roux F, Donnez J. Early-stage endometriosis:adhesion and growth of human menstrual endometrium in nude nude mice [J]. Fertil Steril, 2000, 74(2) :306 - 312.
5Chunhong Yan, Heng Wang, Douglas D. Boy. KISS-1 represses 92-kDa type Ⅳ collagenase expression by down-regulating NF-B binding to the promoter as a consequence of lB-induced block of p65/p50 nuclear translocation [J]. J Biol Chem, 2001,276(2):1164 - 1172
6Sanchez-Carbayo M, C. apodieci P, Cordon-Cardo C. Tumor suppressor role of KISS-1 in bladder cancer: loss of KISS-1 expression is associated with bladder cancer progression and clinical outcome [J]. Am J Pathol, 2003, 162(2) :609 -617.
7Ringel MD, Hardy E, Bemet VJ, et al. Metastin receptor is overexpressed in papillary thyroid cancer and activates MAP kinase in thyroid cancer cells [J]. J Clin Endocrinol Metab, 2002, 87(5):2399 - 2402.
8Ohtaki T, Shintani Y, Honda S, et al. Metastasis suppressor gene KISS-1 encodes peptide ligand of a G-protein-coupled receptor [J].Nature, 2001,411(31):613-617.
9Koks CAM,Groothuis PG,Slaats P,et al.Matrix metalloproteinases and their tissue inhibitors in antegradely shed menstruum and peritoneal fluid[J].Fertil Steril,2000,73:604-612.
10Nap AW,Dunselman GA,de Goeij AF,et al.Inhibiting MMP activity prevents the development of endometriosis in the chicken chorioallantoic membrane model[J].Hum Reprod,2004,19:2180-2187.