摘要
在对国内某地区７个患病猪群流行病学调查的基础上，用Ｍａｒｃ１４５细胞培养从４个猪群流产胎儿分离到３株导致细胞病变的病毒———Ｊ１、Ｊ２和Ｊ３株。它们对氯仿和热（５６℃，１ｈ）、甲醛、酸、碱均敏感。病毒粒子呈球状，直径３０～８０ｎｍ，负染后病毒粒子大小８０～１００ｎｍ，在Ｍａｒｃ１４５细胞质内增殖，５溴２′脱氧尿核苷（ＢＵＤＲ）对其无抑制作用。结合血清学试验，鉴定３个毒株均为猪繁殖和呼吸综合征病毒（ＰＲＲＳＶ），可能为美洲型ＰＲＲＳＶ。
On base of the epizootiology of seven pig herds with porcine reproductive and respiratory syndrome (PRRS) outbreaks in China, three virus strains were isolated from aborted fetues in the herds on Marc 145 cell culture. The three isolates, designated as J1, J2 and J3, could cause cytopathic effects (CPE), which could not be inhibited by BUDR. The causal virus did not resist treatments with chloroform, pH 5.0, pH 8.0 and 56 ℃, and decreased significantly in infectivity when exposed to 0.2% formalin for 2 h at 37 ℃. The diameter of the virus detected in cytoplasm of Marc 145 cells was 30 80 nm, but in negatively stained electron micrographs was 80 100 nm. They showed serological relationship to the American serotype of PRRS virus. The results confirmed that PRRS virus was present in China.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第3期82-86,共5页
Journal of Nanjing Agricultural University
基金
国家自然科学基金
江苏省九五重点攻关项目
关键词
猪病
分离
鉴定
PRRS
porcine reproductive and respiratory syndrome virus
isolation
identification
