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微结构葡聚糖凝胶浓缩分离蛋白质的性能 被引量：2

Protein concentration and separation behavior of dextran bulk hydrogels with microstructure
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摘要 The microstructure,swelling ratio and protein concentration and separation behavior of dextran bulk hydrogels with microstructure(BHMs)were studied with scanning electron microscopy(SEM),visible ultra-violet spectrophotometry(UV)and high performance liquid chromatography(HPLC).The experimental results indicated that the bovine serum albumin concentration and separation efficiency and swelling ratio increased with increasing microgels content or decreasing temperature.Comparing with the cross-linked dextran hydrogels,dextran BHMs realized baseline separation for multi-component proteins including bovine serum albumin,lysozyme and ovalbumin;additionally,the separation time was reduced from 24 h to 9 h.The excellent concentration and separation performance of dextran BHMs were ascribed to its loose and porous microstructure. The microstructure,swelling ratio and protein concentration and separation behavior of dextran bulk hydrogels with microstructure（BHMs）were studied with scanning electron microscopy（SEM）,visible ultra-violet spectrophotometry（UV）and high performance liquid chromatography（HPLC）.The experimental results indicated that the bovine serum albumin concentration and separation efficiency and swelling ratio increased with increasing microgels content or decreasing temperature.Comparing with the cross-linked dextran hydrogels,dextran BHMs realized baseline separation for multi-component proteins including bovine serum albumin,lysozyme and ovalbumin;additionally,the separation time was reduced from 24 h to 9 h.The excellent concentration and separation performance of dextran BHMs were ascribed to its loose and porous microstructure.
作者 徐玉福 姚日生 邓胜松
机构地区 合肥工业大学化工学院
出处 《化工学报》 EI CAS CSCD 北大核心 2007年第12期3097-3101,共5页 CIESC Journal
基金 合肥工业大学科学研究发展基金项目(037513)~~
关键词 微结构 葡聚糖凝胶 蛋白质 浓缩分离性能 microstructure dextran hydrogel protein concentration and separation behavior
分类号 TQ028.8 [化学工程]
  • 相关文献

参考文献15

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共引文献111

同被引文献23

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化工学报
2007年 第12期

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