摘要
以1周龄伊莎雏鸡为实验对象,采用连续腹腔注射的方法,研究了铝暴露对雏鸡免疫功能的影响.实验设对照、低、中、高4个剂量组,暴露剂量分别为0、18.31、27.47、36.62mg·kg-1·d-1(Al3+),暴露60d后,检测雏鸡血清、脾脏和法氏囊中元素Al、Fe、Zn、Mn、Ca、Cu含量及血清补体(C3、C4)、白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)含量.结果表明,暴露60d后,与对照组雏鸡相比,低、中、高剂量组雏鸡血清、脾脏和法氏囊中Al、血清补体C4和TNF-α含量均有不同程度地升高,而Fe、Zn、Mn、Ca、Cu、C3和IL-2含量均有不同程度地降低;随着铝暴露剂量的增加,Al、C4和TNF-α含量逐渐升高,而Fe、Zn、Mn、Ca、Cu、C3和IL-2含量逐渐降低,均存在一定的剂量-效应关系.上述结果表明,铝暴露对雏鸡免疫功能具有明显的抑制作用.
The effects of exposure to aluminium on chicken＇s immune system were studied by continuous peritoneal injection to one-week-old YiSha chickens. Chickens were separated into four groups： control, low-dose, middle-dose, high- dose group, and the doses were 0,18.31,27.47,36.62mg·kg^-·d^-1（Al^3＋）, respectively. After 60d exposure, the contents of Al, Fe, Zn, Mn, Ca and Cu in serum, spleen and bursa of fabricius as well as C3, C4, IL-2 and TNF-α in serum were determined. Results showed that, after 60d exposure, the contents of Al, C4 and TNF-α increased in varying degrees and the contents of Cu, Zn, Fe, Ca, Mn, C3 and IL-2 decreased in varying degrees, compared with the control groups. With the increase of aluminium exposure doses, the contents of Al, C4 and TNF-α increased gradually and the contents of Cu, Zn, Fe, Ca, Mn, C3 and IL-2 decreased gradually and dose-effect relationships were found. Aluminium intoxication had significant inhibitory effects on the immune function in chickens.
出处
《生态毒理学报》
CAS
CSCD
2007年第4期445-449,共5页
Asian Journal of Ecotoxicology
基金
黑龙江省教育厅科研项目(No.11521019)
关键词
雏鸡
铝暴露
免疫功能
chickens
aluminium intoxication
immune function
