摘要
目的:近期研究发现,壳聚糖纳米化后,不仅可改善其溶解性,还可提高其生物学功能。拟建立壳聚糖纳米粒的制备方法,并对壳聚糖纳米粒的表征及抗肿瘤生物学效应进行初步研究。方法:实验于2006-08/2007-05在浙江省医学科学院生物工程所完成。①建立壳聚糖纳米粒的制备方法:将壳聚糖粉末溶于乙酸溶液,用NaOH调节其pH为5,采用三聚磷酸钠为凝聚剂,进行离子交联来制备壳聚糖纳米颗粒。通过离心和冷冻干燥得到壳聚糖纳米粒粉末。②纳米粒的表征:经超声得到壳聚糖的悬浊液,用透射电镜来观察纳米颗粒的外观形态;用动态光散射仪来测定纳米颗粒的粒径大小与分布。③采用MTT法对壳聚糖纳米粒体外抗肿瘤生物学效应进行了初步研究。结果:①透射电镜下观测到了稳定、均一的颗粒;激光粒度分析仪测量发现纳米粒粒径大小在300nm左右,粒径分布较窄。②500mg/L的壳聚糖纳米粒对Hela细胞的抑制率为27%;对SMMC-7721细胞的抑制率为23%;对BGC-823细胞的抑制率为29%;对MCF-7细胞的抑制率最高,达55%。结论:建立的壳聚糖纳米粒的制备方法可靠,并证明其体外具有较好的抗肿瘤作用。
AIM： Recent researches show that, chitosan nanoparticles can ameliorate their dissolubility and improve biological performance. This study was planned to establish a preparation method of chitosan nanoparticles, and primarily investigate the characterization and anticancer effect of chitosan nanoparticles. METHODS： The experiment was performed in the Biological Engineering Institute, Zhejiang Academy of Medical Sciences from August 2006 to May 2007. （1）Preparation of chitosan nanoparticles： Chitosan powder was dissolved in acetic acid and then chitosan nanoparticles was obtained by ionic crosslinking with tripolyphosphate in the pH 5 solution. （2）Characterization of chitosan nanoparticles： Chitosan suspension was obtained by ultrasound. Transmission electron microscopy and Zetasizer instrument were used to detect the shape and size of nanoparticles. （3）A primary study on the anticancer effect of chitosan nanoparticles was analyzed by MTT assay. RESULTS： （1）It was clearly illustrated by transmission electron microscopy that the chitosan nanoparticles had a solid and consistent structure. The size of chitosan nanoparticles was about 300 nm and particle diameter was relatively narrow. （2）The inhibitory rate of 500 mg/L chitosan nanoparticles in Hela cell proliferation was 27%, in SMMC-7721 cell proliferation was 23%, in BGC-823 cell proliferation was 29%, in MCF-7 cell proliferation was the highest, up to 55%. CONCLUSION： The preparation method of chitosan nanoparticles is reliable, and chitosan nanoparticles is proved to show good antitumor activity in vitro.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第48期9688-9691,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
浙江省科技厅重大项目资助(2005F11010)
浙江省卫生高层次创新人才工程资助~~
