摘要
目的:研究显示,胰岛素样生长因子Ⅰ和转化生长因子β1与溃疡性结肠炎患者肠纤维化的形成关系密切。实验通过艾灸对大鼠结肠成纤维细胞分泌胰岛素样生长因子Ⅰ、转化生长因子β1的影响,探索艾灸防治溃疡性结肠炎肠纤维化机制。方法:实验于2004-05/2005-07在上海中医药大学实验动物室和国家中医药管理局针灸免疫三级实验室完成。①实验材料:SPF级雄性SD大鼠75只,体质量200g左右。②实验分组及处理:采用免疫学方法加局部刺激制备溃疡性结肠炎大鼠模型,随机将大鼠分为正常组、模型组、隔药灸组、温和灸组和西药组。隔药灸组、温和灸组选取天枢、气海穴分别进行隔药灸、温和灸治疗,西药组柳氮磺胺吡啶溶液灌胃治疗,模型组和正常组仅固定不做治疗。治疗结束后麻醉下处死大鼠,剖取结肠组织,分离并培养结肠成纤维细胞。③实验评估:用酶联免疫吸附法检测各组大鼠成纤维细胞上清液中胰岛素样生长因子Ⅰ、转化生长因子β1含量。结果:每组取8只进入结果分析。①造模大鼠结肠黏膜缺损,溃疡形成,胶原纤维增生,Ⅰ、Ⅲ、Ⅳ型胶原纤维排列紊乱,数量增多;肉芽组织、纤维组织增生。②模型组大鼠结肠成纤维细胞大量分泌转化生长因子β1、胰岛素样生长因子Ⅰ;与模型组比较,隔药饼灸、温和灸组大鼠转化生长因子β1分泌量减少(P<0.05,P<0.01),隔药饼灸、温和灸和西药组大鼠胰岛素样生长因子Ⅰ分泌量减少(P均<0.01)。结论:艾灸大鼠天枢、气海穴能抑制大鼠结肠成纤维细胞分泌促细胞外基质细胞因子胰岛素样生长因子Ⅰ、转化生长因子β1,减少细胞外基质的积聚,达到防治肠纤维化的作用。
AIM： Previous researches show that, insulin-like growth factor- Ⅰ （IGF- Ⅰ ） and transforming growth factor-β1 （TGF-β1） are closely related to the colonic fibroblasts in ulcerative colitis fibrosis patients. This study investigated the moxibustion＇s effect on regulating the secretion of IGF- Ⅰ and TGF-β1 of colonic flbroblasts in rats to explore the mechanism of treating ulcerative colitis fibrosis by rnoxibustion. METHODS： From May 2004 to July 2005, the experiment was carried out in the Experimental Animal Center and Grade-Ⅲ Laboratory of Acupuncture and Immunity by State Administration of Traditional Chinese Medicine in Shanghai University of Traditional Chinese Medicine.（1）Totally 75 male SD rats （SPF grade, weighting about 200 g） were adopted.（2）A rat model of ulcerative colitis fibrosis was established by immunological method and local stimulation. All the model rats were randomly divided into model group, herbs-partition moxibustion group, mild-warm moxibustion group and salazopyrin （SASP） group. Normal rats were used as normal control group. Herbs-partition moxibustion group and mild-warm moxibustion group were treated by herbs-partition moxibustion and mild-warm moxibustion respectively on Qihai （RN 6） and Tianshu （ST 25） points. SASP group was fed with medicine SASP. Normal control group and model group gave no treatment. After the treatment all the rats were killed under anesthesia, and colonic tissues were harvested. Colonic fibroblasts from all the rats were isolated and cultured. （3） IGF- Ⅰ and TGF-β1 contents in the supernatants of different groups were determined by enzyme linked immunosorbent assay. RFSULTS： Eight rats from each group were involved in the result analysis.（1）There were damages, ulcers and collagen fibrous hyperplasia in the colon mucosa membrane of model rats, with collagen Ⅰ , Ⅲ and Ⅳ ranked disorder and increased. Both granulation tissue and fibrous tissue appeared hyperplasy,（2）TGF-β1 and IGF- Ⅰ secretion from colonic fibroblasts of rats in model group increased. Treatment of herbs-partition moxibustion and mild warm moxibustion decreased the secretions of TGF-β1 （P 〈 0.05, P 〈 0.01）, and IGF- Ⅰ content was reduced in herbs-partition moxibustion group, mild-warm moxibustion group and SASP group （P 〈 0.01）. CONCLUSION： Moxibustion on Qihai （RN 6） and Tianshu （ST 25） points is effective in decreasing the secretion of TGF-β1 and IGF- Ⅰ colonic fibroblasts in rats, reducing the accumulation of extracellular matrix, and preventing colonic fibrosis in ulcerative colitis rats.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第49期9878-9881,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金项目资助(30400609)
上海市青年科技启明星项资助目(06QA14049)
上海市重点学科建设项目资助(T0302)~~
