摘要
目的观察微孔聚甲基丙烯酸-β-羟乙酯[Poly(β-hydroxyethyl methacrylate)],PHEMA和微孔聚甲基丙烯酸甲酯(polymethyl methacrylate,PMMA)材料植入干眼模型兔角膜层间后引起的组织学和超微结构的改变,评价这两种材料的生物相容性,为进一步的研究提供依据。方法干眼模型兔12只,随机分为2组,分别在右眼造模眼的角膜层间植入微孔PHEMA、微孔PMMA材料,分别于术后0.5月、1月、2月处死动物,行HE染色光学显微镜检查和透射电镜检查。结果实验兔的眼部刺激症状,均在术后短期内迅速缓解;术后0.5月,材料的孔隙内可见少量长梭形的角膜基质细胞和成纤维细胞;术后2个月,角膜基质细胞和成纤维细胞继续长入材料的孔隙内并向材料的中心迁徙,同时合成胶原纤维。结论微孔PHEMA、微孔PMMA材料植入干眼模型兔角膜层间后对组织刺激性较小,具有良好的生物相容性。
Objective To study the changes of corneal histology and ultrastructure after implantation of Poly （2-hydroxyethyl methacrylate （PHEMA） and Polymethyl methacrylate （PMMA） in dry eye rabbit corneal stroma and to evaluate their biological compatibility, Methods Twelve dry rabbit eyes were divided into two groups randomly. Disc of microporous PHEMA or PMMA was implanted into the right cornea respectively. Rabbits were killed after 0.5,1,2 months of surgery to examine the excised cornea by light microscopy and transmission microscopy. Results Within one week after surgery, the symptoms of experimental eyes were ameliorated evidently. By two weeks after implantation, corneal fibroblast cells and deposition of collagens were found in the pores of PHEMA and PMMA materials, which migrating forward to the center of materials gradually in next 2 months. Conclusion PHEMA and PMMA have favorable biological compatibility in dry eye models, which permitting corneal fibroblast cells ingrowig growing and collagens depositing into the pores.
出处
《同济大学学报（医学版）》
CAS
2007年第6期12-16,共5页
Journal of Tongji University(Medical Science)
