摘要
提出了用高效液相色谱法测定藻类中类胡萝卜素和叶绿素的方法。采用丙国等有机溶剂提取藻类中的类胡萝卜素和叶绿素，然后在反相C18柱上进行分离。流动相选用二氯甲烷／乙腈／甲醇／水（22.5:9.5:67.5:0.5），流速为1.0mL／min。用光度检测器检测报长为450um。叶黄素、α-胡萝卜素、β-胡萝卜素、叶绿素a和叶绿素b的平均回收串分别为99.1％，98.5％，99.4％，100.6％和99.9％，相对标准偏差分别为2.4％，5.6％，6.0％，4.1％和4.0％。
An isocratic reversed-phase HPLC method for the simultaneous determination of carotenoids and chlorophylls in algae is presented. Both carotenoids and chlorophylls were separated by using a C18 column and a mobile phase consisting of d ichloromethane/acetonitrile/methanol/water (22.5 : 9.5: 67. 5:0.5 ). The flow rate was 1. 0mL/min. These pigments were detected by a UV-VIS absorbance detector set at 450nm. The average recoveries of these pigments by standard addition method were 99. 1% for lutein,98. 5 % for a- carotene, 99. 4% for β- carotene, 100. 6% for chlorophyll a and 99. 9% for chlorophyll b. The relative standard deviations of these pigments were 2. 4% for lutein, 5. 6% for α- carotene, 6.0% for β-carotene, 4. 1 % for chlorophyll a and 4. 0% for chlorophyll b. The calibration curves for these pigments gave good linearity over a wide range of o-50mg/L (r=0.9992-0. 9999), The retention times were 3.0 min for lutein, 4. 7 min for chlorophyll b, 6. 3 min for chlorophyll a, 14. 1 min for α- carotene, and 14. 7 min for β-carotene. Using this method, carotenoids and chlorophylls were determined in Spirulina Platensis, Chlorella vulgaris and Haematococcus lacustris. Chlorella vulgaris and Haematococcus lacustris samples were ground in a B. Braun homogenizer. Spirulina platensis sample was smashed by supersonic wave, Acetone was used as extractant.
出处
《色谱》
CAS
CSCD
北大核心
1997年第2期133-135,共3页
Chinese Journal of Chromatography
基金
香港RGC和香港大学CRCG资助
关键词
高效液相色谱法
类胡萝卜素
叶绿素
藻类
high performance liquid chromatography, carotenoids, chlorophylls, algae
