摘要
报告了用RP-HPLC测定免血清和脑脊液中甲硝唑浓度的方法，采用ZorbaxC8柱，NaH2PO4水溶液（0.0075mol／L）-甲醇（4:1，PH4.0）为流动相，氢氯噻嗪为内标，检测波长320um。线性范围0.5～80mg／L，最低检出浓度80μg／L（信噪比为3）。
A reversed-phase high performance liquid chromatographic method for the determination of metronidazole (MTZ) in rabbit serum and cerebro-spinal fluid has been developed. The method is simple and convenient.The analysis was carried out with Zorbax C8 column,NaH2PO4 (0.0075mol/L,pH 4. 0)-CH3OH(4: 1 ) as mobile phase and UV detector at 320nm,by using hydrochlorothiazide as internal standard. The standard curve is linear over the range from 0. 5ng/L to 80. 0ng/L in sample with correlation coefficient of 0. 9998. The recovery of metronidazole from rabbits serum and cerebrospinal fluid are 97. 1 % ± 1. 5 % and 97. 4% f 1. 6 % respectively.The minimum detectable quantity is 80ng/mL in sample(S/N = 3 ). Under the chromotographic condition,the consistency of MTZ of the serum and cerebrospinal fluids of rabbits were detected separately after taking MTZ (0. 1mg/g) orally. The drug-time curve was obtained and a satisfactory result was achieved. The new findings provide conditions for further exploration of the process of pharmacokinetics.
出处
《色谱》
CAS
CSCD
北大核心
1997年第2期170-172,共3页
Chinese Journal of Chromatography
关键词
高效液相色谱法
甲硝唑
血清
脑脊液
reversed-phase high performance liquid chromatography, metronidazole, rabbit serum, rabbit cerebrospinal fluid
