摘要
目的 建立操作简便，与人体肝癌发生发展过程相似的肝癌动物模型。方法 取体重150～200g雄性Wistar大鼠150只，随机分为实验组和对照组，实验组饲以含二乙基亚硝胺（DEN，80×10^-6）饮水，连续12周后改为自由饮水。对照组常规自由饮水。结果 病理学检查证实DEN诱发的肝癌为肝细胞癌，诱癌率为70．6％（36／51），大鼠肝癌癌变过程大致经过肝细胞损伤期、肝细胞增生．硬化期和肝细胞癌变期等三个阶段。结论 饲以低剂量DEN诱发大鼠肝癌模型建立成功率高，是一种较理想的研究人体肝癌发生的动物模型。
Objective To establish an experimental hepatocellular carcinoma rat model which is convenient and similar to the human hepatocarcinogenesis. Methods one hundred and fifty male Wistar rats, weighing 150 to 200g, were randomly divided into two groups. The experimental group＇s drinking water contained 80ppm diethylnitrosamine for 12 continuous weeks. The control drank the routine table-water. Results 70. 6% （36/51 ） hepatocellular carcinoma were induced by DEN. The procession of hepatocarcinogenesis in this model included three stages-hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. Conclusion The successful rate of liver cancer model induced by low dose DEN was high, it is an ideal experimental model for the study of hepatocarcinogenesis.
出处
《滨州医学院学报》
2007年第6期401-403,406,共4页
Journal of Binzhou Medical University
基金
山东省自然科学基金资助课题(Y2005C40)
关键词
二乙基亚硝胺
肝细胞癌
大鼠
动物模型
Diethylnitrosamine, hepatocellular carcinoma, rat,animal model
