摘要
目的研究色氨酸羟化酶(tryptophan hydroxylase,TPH)基因座-T457C位点在中国北方汉族群体中的遗传多态性及其法医学应用价值。方法应用PCR-RFLP、聚丙烯酰胺凝胶电泳及银染技术,检测180例北方汉族无关个体TPH基因座-T457C位点的遗传多态性。结果TPH基因座-T457C位点在中国北方汉族群体中,其个体识别能力(DP)为0.624,非父排除率(PE)为0.187,与法国人群基因频率比较差异显著(P<0.05)。结论TPH基因座-T457C位点在法医学中有重要应用价值,其遗传多态性可能存在民族或地域差异。
Objective To study the distribution of the tryptophan hydroxylase （TPH） gene-T457C locus polymorphism in Han ethnic group in northern China and to find its applicable value in forensic science. Methods Genomic DNA samples, extracted from 180 unrelated individuals in northern Chinese Hart population, were analyzed by PCR-RFLP. Results The discrimination power （DP） value and the power of exclusion （PE） value of the TPH gene-T457C locus were 0.624 and 0.187, respectively. The allele frequency showed significant difference from that of French people （P=0.04）. Conclusion Polymorphism of the TPH gene-T457C locus could show ethnic and regional differences. It has a potential to be used in forensic science.
出处
《法医学杂志》
CAS
CSCD
2007年第6期444-445,共2页
Journal of Forensic Medicine
基金
辽宁省教育厅高等学校科研项目(2004D052)
关键词
法医学
TPH
单核苷酸多态性
forensic science
tryptophan hydroxylase （TPH）
single nucleotide polymorphism
