摘要
目的测定胃乐胶囊中甘草次酸的含量。方法用反相高效液相色谱(RP-HPLC)法对方中的主要有效成分甘草次酸进行含量测定,色谱柱为C18柱,流动相为乙腈-0.5%冰醋酸(70∶30),检测波长250nm。结果甘草次酸质量浓度在4.992～249.6mg/L范围内与峰面积线性关系良好,回归方程Y=3.0×107X+5.9×103,r=0.9997;平均加样回收率为99.18%,RSD为1.89%(n=6)。结论RP-HPLC法操作简单,重现性好,测定结果准确可靠。
Objective To establish a method for determining glycyrrhetinic acid in Weile Capsule. Methods The method of RP- HPLC was used. C18 column was adopted with UV detector. The mobile phase was acetonitrile-0.5% glacial acetic acid （70：30）, the detection wavelength was at 250 nm. Results A good linear correlation was obtained over the range of 4. 992-249. 6 mg/L for glycyrrhetinic acid. The regression equation was Y= 3.0 × 10^7X ＋ 5.9 × 10^3, r = 0. 999 7. The average recovery rate was 99. 18% , RSD = 1.89% （ n = 6）. Conclusion The method is simply operated with good recurrence and accuracy for result.
出处
《中国药业》
CAS
2008年第1期12-13,共2页
China Pharmaceuticals
