摘要
以体外培养人脐静脉内皮细胞系EA.hy926为研究模型,从细胞水平上分析了血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)损伤过程及中药川芎的一种有效成分——盐酸川芎嗪(tetramethylpyrazine hydrochloride,TMP·HCl)保护过程中内皮细胞的相关酶活性变化及信号转导规律。结果表明,体外培养内皮细胞在AngⅡ刺激下,细胞活力降低;且ERK、JNK的磷酸化程度,内皮型一氧化氮合成酶(eNOS)和诱生型一氧化氮合成酶(iNOS)表达量,胞内抗氧化酶的活力分别发生变化;而TMP·HCl预保护后,发生相反变化,同时提高了细胞活力,证实ERK、JNK、eNOS、iNOS等信号分子以及活性氧参与细胞损伤及存活过程。
We set human umbilical vein endothelial cell line EA.hy926 as a model toanalyze the activity changes of relative enzymes and regulation mechanism of relative signal pathways of the model under the condition of injury by angiotensin Ⅱ （AngⅡ） and / or protection by tetramethylpyrazine hydrochloride （TMP·HCl）, an effective component of traditional Chinese medicine Ligusticum chuanxiong Hort. Results showed that viability of cultured endothelial cell decreased after stimulating by AngⅡ, while phosphorylation level of ERK and JNK expression, amount of eNOS and iNOS, activity of SOD and CAT altered. Pretreatment by TMP·HCl reversed all these changes and increased the cell viability, which demonstrated that ERK, JNK, eNOS, iNOS and ROS play important roles in the process of cell injury and survival.
出处
《细胞生物学杂志》
CSCD
2007年第6期895-900,共6页
Chinese Journal of Cell Biology
基金
国家杰出青年基金资助项目(No.20425620)~~
