摘要
目的构建子宫内膜异位种植裸鼠模型,在此基础上研究抗血管内皮生长因子(VEGF)抗体在治疗中的作用及机制。方法将人子宫内膜组织种植到裸鼠体内,抗VEGF抗体作用于种植灶,进行分组对照研究。用TUNEL标记法检测细胞凋亡、PCNA检测细胞增殖和微血管密度(MVD)。结果实验组细胞凋亡强度明显高于对照组,细胞增殖强度在各组间无明显差异。实验组人源性MVD和鼠源性MVD都明显低于对照组(P<0.05)。结论抗VEGF抗体可能是通过促进子宫内膜细胞和血管内皮细胞的凋亡、抑制血管生成,继而抑制内膜异位种植生长,而对细胞增殖无明显抑制作用。
Objective To establish the nude mouse model for in vivo research on endometriosis. So to study the mechanism and effect of anti-vascular endothelial growth factor （VEGF） antibody treatment on the growth of established endometriotic-like lesions in the nude mouse model. Methods Human endometrium is implanted into nude mice and used to test the effect of anti-VEGF antibody. The models were seperated into control groups and experimental groups （using anti-VEGF antibody）. The TUNEL, PCNA and MVD were used to evaluate the effects of apoptosis, proliferation and angiogenesis. Results The explants in the control groups develop a rich blood supply that enables them to survive and grow than those in the experimental groups. The apoptosis in experimental groups was significantly higher than those of the control groups. The proliferation didn＇ t show difference in these groups. The MVD in the control groups （human non-endometriosis 12. 80 ±4.60, endometriosis 13.15 ± 5.66 ; mouse non-endometriosis 29.7 ± 19.6, endometriosis 34. 6 ± 16.3 ） were higher than those in the experimental groups （ human non-endometriosis 7. 17 ± 2.25 ; endometriosis 7. 32 ± 1.30 ; mouse non-endometriosis 11.2 ± 6. 2 ; endometriosis 15.6 ±6.8）. Conclusion Anti-VEGF antibody effectively interfered with the maintenance and growth of endometriotic-like lesions by disrupting the vascular supply. The anti-VEGF antibody may be provided a novel therapeutic approach for the treatment of endometriosis.
出处
《基础医学与临床》
CSCD
北大核心
2007年第12期1360-1364,共5页
Basic and Clinical Medicine
