摘要
[目的]研究仁用杏的抗逆性机理。[方法]以5年生发育良好的仁用杏苗木的叶片为试验材料,于硬化期、生长初期和速生期对仁用杏苗木进行铜、锌喷雾处理,采用NBT还原法和邻苯二酚法分别对仁用杏叶片的多酚氧化酶(PPO)活性和超氧化物歧化酶(SOD)活性进行测定,分析了仁用杏叶中主要保护酶PPO和SOD的酶活性在不同生育时期的变化规律。[结果]仁用杏叶经Cu(3)Zn(0)处理后PPO活性显著提高,且在硬化期也保持较高的水平。仁用杏叶经Cu(0)Zn(3)处理后PPO活性的增强很小甚至表现负作用。仁用杏叶经过Cu(0)Zn(3)处理后SOD的活性有显著提高,生长初期的SOD活性明显高于硬化期和速生期。[结论]锌对仁用杏叶内SOD的活性的增强作用比较显著,铜、锌通过PPO和SOD的活性的改变影响仁用杏苗木生长,从而提高苗木抗逆性。
Objective The purpose was to study the stress resistance mechanism of Kernel-apricot.Method With leaves collected from developed well 5-year-old Kernel-apricot seedlings as tested materials,the seedlings were treated with copper and zinc spraying in their hardening,initial growing and fast-growing stages.The activities of superoxide dismutase（SOD）and polyphenol oxidase（PPO）in Kernel-apricot leaves were determined by NBT reduction method and pyrocatechol method.The change laws of main protective enzyme PPO and SOD activity in Kernel-apricot leaves in different growth periods were analyzed.Result The PPO activity in Kernel-apricot leaves treated with Cu（3）Zn（0）increased significantly and was also kept at higher level in hardening stage.Treating Kernel-apricot leaves with Cu（0）Zn（3）increased PPO activity very little,even had decreasing effect it.The SOD activity in Kernel-apricot leaves treated with Cu（0）Zn（3）was increased significantly,and being obviously higher in the initial growth stage than in the hardening and fast-growing stages.Conclusion Zinc has more significant strengthening effect on SOD activity in Kernel-apricot leaves.Copper and zinc affect the growth of Kernel-apricot seedlings through changing PPO and SOD activities,accordingly increase the stress resistance of seedlings.
出处
《安徽农业科学》
CAS
北大核心
2007年第35期11370-11371,11374,共3页
Journal of Anhui Agricultural Sciences
基金
石河子大学高层次人才引进基金项目--自然科学基金项目(项目编号5006-822017)
关键词
仁用杏
多酚氧化酶
超氧化物歧化酶
Kernel-apricot
Polyphenol oxidase（PPO）
Superoxide dismutase（SOD）
