摘要
目的:研究中国环境中新生隐球菌变种的生态学特点。方法:采集10个城市(3组)鸽粪标本620份和江西贵溪地区澳洲赤桉标本819份;采用咖啡酯玉米山梨醇琼脂(CACA)培养基分离新生隐球菌,并对菌株进行CGB培养,酚氧化酶、尿素酶、血清型、交配型试验和形态学观察。结果:①不同纬度地区鸽群的新生隐球菌标本阳性率为G1[40°～50°北纬(N)]13%,G2(30°～40°N)50%,G3(20°～30°N)29%,G2显著高于G1(P<0.01);3组鸽群阳性率基本相同(P>0.05),平均为81%。②环境分离358株全部37℃生长、酚氧化酶阳性、CGB培养阴性;1株尿素酶阴性;其中101株均为α交配型和A血清型,提示全部为grubii变种;发现环境分离菌株荚膜变异并与其他真菌的自然黏附现象。③从江西贵溪生长的澳洲赤桉未分离到新生隐球菌格特变种。结论:中国环境中以grubii变种为主的新生隐球菌的生态学特点与其所在的地区纬度明显相关;由澳洲赤桉分离新生隐球菌格特变种的研究仍然需要继续进行。
Objectives： To study the ecology of the varieties of Cryptococcus neoformans in China. Methods： Six hundred and twenty pigeon dropping samples from 10 cities and 819 environmental samples related to the Eucalyptus camaldulensis trees growing in Guixi area of Jiangxi province were collected and inoculated on the caffeic acid cornmeal agar. The isolates were studied by the following techniques or treatment： growing on CGB culture, phenol oxidase, urease, serotypes, mat- ing types as well as morphology. Results： It was found that the isolation rates of C. neoforrmms from pigeon droppings in different regions of different latitudes were different： 50% in the northern latitude 30°~40°cities, 29% in the N 20°~30° cities and 13% in the N 40°~50° cities. However, there was no difference in the positive rates in three pigeon bevies. All mycological tests showed that 101 isolates from pigeon droppings were C. neoformans var. grubii （serotype A, α mating type and no growth on CGB medium）. Also, a natural adhesion phenomenon of C. neoforrmms cells with other yeasts was ob- served. No C. neoforrmms vat. gattii were isolated from all 819 samples of E. camaldulensis trees. Conclusions： The ecologi- cal features of C. neoformans vat. grubii in China significantly corrected with its latitude distributions. It is still needed to isolate C. neoformans vat. gattii from E. camaldulensis trees in more Chinese areas.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2008年第1期3-6,共4页
Journal of Clinical Dermatology
基金
国家自然科学基金资助项目(39670044)
关键词
新生隐球菌
环境分离
生态学
Cryptococcus neoformans
environmental isolation
ecology
