摘要
目的修订玉屏风颗粒中黄芪甲苷的含量测定方法。方法高效液相色谱法，以十八烷基硅烷键合硅胶为填充剂，以乙腈-水（32：68）为流动相；蒸发光散射检测器。结果黄芪甲苷在0．25—5．0μg范围内线性关系良好；平均回收率为99.9％，RSD=2．6％（n=6）。结论方法简便，重复性好，适合作为玉屏风颗粒的含量测定方法。
Objective To revise the method of determination of the contents of astragaloside in Yupingfeng Granules. Methods The determination was performed by HPLC-ELSD using C18 column,CH3CN-H2O （32： 68） as mobil phase. Results There was a good linear relationship between the logarithmic of the absorption area and the logarithmic of the concentration in the range of 0.25 - 5.0 μg for astragaloside. The average recovery of standard addition were 99. 9%, RSD = 2.6 % （ n = 6 ）. Conclusion The method is simple, accurate, and reproducible, and can be used for content determination of Yupingfeng Granules.
出处
《现代食品与药品杂志》
2007年第6期40-44,共5页
JOurnal of Modern Food and Pharmaceuticals
