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副猪嗜血杆菌PCR鉴定方法的建立 被引量:8

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摘要 以HPS的16SrRNA中的基因序列设计合成引物,进行PCR扩增,标准菌株NR4、SW124、SW114、SW140和分离株XX、FS在预计的821bp位置上扩增出特异性条带,NJING株、胸膜肺炎(APP)等没有特异性条带产生。这证明PCR检测副猪嗜血杆菌具有较高的特异性和敏感性。
出处 《猪业科学》 2007年第12期26-27,共2页 Swine Industry Science
基金 江苏省科技成果转化专项资金(BA2004032) 江苏省自然科学基金重点项目(BK2007711)资助。
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参考文献6

  • 1张培君,孙惠玲,苗得园,龚玉梅,Pat Blackall.猪胸膜肺炎放线杆菌和副猪嗜血杆菌的鉴别诊断及血清型鉴定[J].中国兽药杂志,2002,36(10):18-20. 被引量:27
  • 2Rafriee M, Bara M, Srephens C P, Blackall P J. Application of ERIC-PCR for the comparison of isolates of Haemophilus parasuis[J]. Aust Vet J. 2000,78(12):846-849.
  • 3Hill C E, Metcalf D S, MacInnes J I.A search for virulence genes of Haemophilus parasuis using differential display RT-PCR[J]. Vet Microbiol,2003, 96(2): 189-202.
  • 4Puente-Redondo V A, Blanco N G.Gutierrez-Martin Detection and subtyping of Actinobacillus pleuropneumoniae strains by PCR-RFLP analysis of the tbpA and tbpB genes[J]. Res Microbiol 2000,151(8): 669-681.
  • 5Amano H,Shibata M, Kajio N,Morozumi. Pathologic observa tions of pigs intranasally inoculated with serovar 1,4 and 5 of Haemophilus parasuis using immunoperoxidase method[J]. J Vet Med Sci,1994, 56(4):639-644.
  • 6Jung K.Chae C.Hybridization for the detection of Haemophilus parasuis in naturally infected pigs[J]. J Comp Pathol, 2004,130 (4): 294-298.

二级参考文献6

  • 1[1]Gram Trine. Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein[J]. Journal of Clinical Microbiology, 1998, 36(2):443-448.
  • 2[2]Blackall Pat, H.L.B.M. Klaasen, H. Van Den Bosch, et al. Proposal of a new serovar of Actinobacillus: serovar 15[J]. Veterinary Microbiology, 2002, 84: 47-52.
  • 3[3]Kielstein Peter and Rapp-Gabrielson VJ. Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heatstable antigen extracts[J]. Journal of Clinical Microbiology, 1992, 30(4):862-865.
  • 4[4]Simone Oliveira, Lucina Galina and Carlos Pijoan. Development of a PCR test to diagnose Haemophilus parasuis infections[J]. Journal of Veterinary Diagnostic Investigation, 2001, 13:495- 501.
  • 5[5]Reid G. G.and P. J. Blackall. Comparison of adjuvants for an inactivated infections coryza vaccine[J]. Avian Diseases, 1987, 31:59-63.
  • 6[6]Rafiee Main and Blackall P.J. Establishment, validation and use of the Kielstein-Rapp-Gabrilson serotyping scheme for Haemophilus parasuis [J]. Anstralian Veterinary Journal, 2000, 78:172-173.

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