摘要
目的:探讨中药藜芦(Veratrum nigrum L.)的炮制机理研究。方法:利用HPLC-UV方法测定生物碱藜芦新碱(veratrosine)在生品和炮制品中的含量差异以及利用急性毒性实验,考察生品和炮制品毒性差异。结果:藜芦新碱(veratrosine)质量浓度在0 mg/L^2000 mg/L内与峰面积呈良好的线性关系,r=0.9998(n=7);平均回收率为98.6%,RSD为3.17%(n=5)。藜芦水提物灌胃给小鼠的LD50为4.2 g/kg,95%可信限为3.4 g/kg^5.4 g/kg;米泔制藜芦水提物灌胃给小鼠的LD50为5.3 g/kg,95%可信限为4.8 g/kg^6.1 g/kg。醋制藜芦水提物灌胃给小鼠的LD50为3.2 g/kg,95%可信限为2.5 g/kg^3.9 g/kg。结论:藜芦醋制后会使生物碱藜芦新碱(veratrosine)溶出率增高,毒性增强;藜芦米泔制后会使生物碱藜芦新碱(veratrosine)溶出率降低,毒性减弱。
Objective： To study the mechanism of processing Veratrum nigrum L. Methods： By HPLC-UV, the contents of veratrosin in processed and crude dried roots and rhizomes of Veratrum nigrum L. were determined. And the toxicities-of processed and crude dried roots and rhizomes of Veratrum nigrum L. were examined by acute toxicity test. Results： The calibration curve was linear over the range of 0 mg/L -2 000 mg/L with correlation coefficient of 0. 9998 （ n = 7）. The average recovery was 98.6% with RSD of 3.17% （ n = 5）. LD50 of water extract of crude drug of Veratrum nigrum L. （A） on mice by lavage was 4.2 g/kg with 95% confidence level of 3.4 g/kg - 5.4 g/kg; LDs0 of water extract of drug processed with mixture of rice flour and water of Veratrum nigrurn L. （B） on alice by lavage was 5.3 g/kg With 95% confidence level of 4.8 g/kg - 6.1 g/kg. LD50 of water extract of drug processed with rice vinegar of Veratrum nigrum L. （C） on mice by lavage was 3.2 g/kg with 95% confidence level of 2.5 g/kg - 3. 9 g/kg. Conclusion： Concentration of veratrosin in the sample （C） is more than those in the sample （B） and sample （A）, and toxicity of the sample （B） is less than those of the sample （A） and sample（C）.
出处
《河南大学学报（医学版）》
CAS
2007年第4期1-5,共5页
Journal of Henan University:Medical Science
基金
新世纪优秀人才支持计划(NO.NCET-04-0289)
