摘要
目的观察NaF对原代培养猪甲状腺细胞的毒性效应,并探讨其作用机制。方法采用40mg/L,80mg/L和160mg/LNaF对原代培养猪甲状腺细胞进行染毒,48h后光镜下观察猪甲状腺细胞形态结构的变化,并采用吖啶橙染色法,在荧光显微镜下观察细胞核形态结构的变化;采用MTT法测定细胞存活率。结果中、高剂量氟组甲状腺细胞滤泡结构渐消失,细胞变小、变圆,亮度增加。甲状腺细胞核内可见致密浓染的黄绿色染色,呈圆形、月牙形,可见发泡现象及凋亡小体,且细胞的存活率显著低于对照组(P<0.01)。结论NaF对原代培养猪甲状腺细胞具有毒性效应,并呈现一定的剂量效应关系。
Objective To observe the cytotoxic effects of Sodium Fluoride （NaF） on primary porcine thyrocytes and to explore its mechanism. Methods The primary porcine thyrocytes were incubated in 40mg/L, 80mg/L, and 160mg/L sodium fluoride for 48h. The morphology of the ceils and their nucleus stained in acridine orange were observed by light microscope and fluorescence microscope respectively. The cell survival ratio was measured using MTT method, Results The follicle structure disappeared gradually and the cells became small, round and light in the middle and high fluoride groups, It was thus clear that pyknohyperchromic flavo - green tincture was round or crescent in a part of cell nucleus. The major changes included frothing - up phenomenon and withered bodies. The cell survival ratio in the middle and high fluoride groups were markedly lower than the comparison group （ P 〈 0. 01 ）. Conclusions NaF has a significant cytotoxic effect on primary porcine thyrocytes, and there exists dose - related effect to some extent.
出处
《辽宁医学院学报》
CAS
2007年第5期10-11,I0001,共3页
Journal of Liaoning Medical University (LNMU) Bimonthly
基金
辽宁省锦州市科技局资助项目
编号:05B1E07。
