摘要
新抗生素B3543生产菌的菌丝在1号和2号培养基上生长最佳,用溶壁酶酶解经洗涤和过滤纯化,可制得纯度在94%以上的原生质体,其再生率在77%以上。在314株再生株中获得1株高产菌株R203。
The mycelium of new antibiotic B 3543 producer grew well on medium No. 1 and No. 2. The protoplast purity over 94% was prepared after being treated with lysowallzyme and washed and purified by filtering. Their regenerative ratio was above 77%. One high potency strain R 203 was screened from 314 regenerative strains, its productivity was as high as 6500 U/mL after being transferred for several generations on slant.
出处
《湖北农业科学》
1997年第4期34-37,共4页
Hubei Agricultural Sciences
基金
国家科技攻关项目