摘要
目的研究五溴联苯醚(BDE-99)对大鼠甲状腺激素水平及组织结构影响的时间效应。方法21日龄SD大鼠检疫一周后,以60mg/kg的剂量经口给予BDE-99,分别染毒10d、15d、20d、30d、40d,对照组给予相同体积的玉米油处理相同的时间。用放射免疫方法测血清甲状腺激素含量,HE染色观察甲状腺组织结构改变。结果血清FT4、FT3和TSH的浓度第10d时未发现变化;第15d、20d时FT4、FT3水平下降,伴有TSH水平升高;第30d、40d时FT4、FT3和TSH的浓度又恢复正常。组织病理学观察发现,第15d时甲状腺滤泡上皮增生成复层;第20d甲状腺泡上皮继续增生,有的滤泡局灶性发展到3～4层的增生斑;第30d、40d时已可见实心芽和实心继发滤泡。结论BDE-00染毒SD大鼠10d后,血清FT4、FT3水平下降,TSH水平升高,BDE-99对大鼠甲状腺组织的影响存在着时间效应关系。
Objective To study the time-dependent effects of pentabrominated diphenyl ethers on thyroid hormones and the histological structure of thyroids in rats. Methods One hundred healthy 4-week-old SD rats were randomLy divided into 10 groups. The rats in the experimental groups were orally administered with pentabrominated diphenyl ethers （BDE-99） at a dose of 60 mg/kg for 10,15,20,30 and 40 days respectively, while the rats in the control groups received equal volumes of corn oil for the same periods of time. Serum FT4, FT3 and TSH were measured by radioimmunoassay. Sections of thyroids were dyed with hematoxylin-eosin （HE） to detect pathological alterations in thyroid tissues. Results Decreased serum FT4 and FT3 and increased TSH were detected in the BDE-99 treated rats at D15 and D20. No significant differences of serum hormones were found between the experimental and control groups at D10, D30 and D40. The thyroid follicle epithelium of the BDE-99 treated rats proliferated into multiple-layer structure at D15. At D20, proliferative plaque formed in local areas. Solid buds and follicles secondary to the solid buds were detected at D30 and D40. Conclusion BDE-99 leads to structural changes of thyroids, reduces serum FT4 and FT3 and increases TSH. The time-dependent effects are evident in a damage and compensate pattern.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2008年第1期98-100,共3页
Journal of Sichuan University(Medical Sciences)
基金
重庆医科大学创新基金(CX200308)
关键词
五溴联苯醚
甲状腺激素
组织结构
时效关系
Pentabrominated diphenyl ethers（BDE-99） Thyroid hormones Histological structure Time-dependent effect
