摘要
本文对近20年来魔芋生物技术研究取得的进展进行了系统的回顾分析。组织培养是当前魔芋生物技术研究的主要内容,魔芋离体植株再生以器官发生途径为主,包括不定芽和拟球茎两种途径,后者是当前研究的热点。利用组织培养进行有用突变体的筛选和种质资源的保存也取得了一些有价值的结果。以抗病和品质改良为目的的转基因技术取得了较快发展,如抗病基因和抗除草剂基因等已实现成功转化。此外,本文还分析了魔芋生物技术研究中存在的主要问题并提出了相应的对策。
This article reviews the progress in biotechnology research and development ofAmorphophallus Blume （konjac） in the past 20 years. At present, tissue culture is a major part of the konjac biotechnology. In vitro plant regeneration occurs mainly via organogenesis, which consists of bud or cormlet formation; the latter is the focal point of current research. Useful results have been obtained in the selection of useful somaclonal variation and germplasm conservation by konjac tissue culture. In addition, rapid progress has been made in genetic transformation, with the aim of improving disease resistance and quality. Disease- and herbicide-resistant genes have been successfully transformed into konjac plants. Meanwhile, problems remaining for biotechnology research and development are discussed, and the solutions pertinent to the problems are also put forward.
出处
《植物学通报》
CSCD
北大核心
2008年第1期14-19,共6页
Chinese Bulletin of Botany
基金
国家科技攻关项目(No.2003BA901A05)
河南农业大学博士启动基金项目(No.30400247)
关键词
魔芋属
遗传转化
离体保存
突变体筛选
组织培养
Amorphophallus Blume, genetic transformation, in vitro germplasm, somaclonal variation, tissue culture
