摘要
在（23±0.2）℃海水温度下，测定了MS-222对半滑舌鳎（Cynoglossus semilaevis Günther）成鱼的麻醉效果。实验用半滑舌鳎成鱼体长25-40cm，体质量300～1500g。实验设计的MS-222质量浓度范围分别为10～120mg／L和90-240mg／L。结果显示，鱼体在最终麻醉程度不超过3期时呼吸频率增加，最终麻醉程度达到4期以上呼吸频率开始下降。MS-222麻醉半滑舌鳎的有效质量浓度为120～210mg／L。在此浓度范围内，鱼体能够在3min之内达到4期麻醉状态，5min之内苏醒恢复，且在MS-222溶液中浸浴15min后成活率为100％。鱼体在180mg／L的MS-222溶液中麻醉5min，在空气中进行暴露0～21min内，复苏时间相差不大，为100s左右。5尾半滑舌鳎在180mg／L的MS-222溶液中麻醉5min，然后在空气中暴露3min，连续麻醉6次，每次间隔24h，复苏时间差异不显著。实验证明，MS-222麻醉半滑舌鳎效果好，鱼体入麻时间短、复苏快，安全边界宽，反复麻醉没有明显的耐受性，是一种理想的水产用麻醉剂。文中还对MS-222用于半滑舌鳎麻醉运输及人工授精等进行了探讨。
Anaesthetic has been used in aquaculture to minimize the stress and damage of fish during harvesting, grading, transportation, spawning induction and handling. This study investigated the anaesthetic effect of MS-222 on adult Cynoglossus semilaevis GUnther at water temperature （23 ± 0.2） ℃. The body weight and body length of the fish were 300-1 500 g and 25-40 cm, respectively. The concentration of MS-222 was designed at two ranges, which were 10-100 mg/L and 90-240 mg/L. The breath frequency offish increased slightly when the fish were in the final anaesthetic stages 1-3, and declined clearly until stage 4 within 3 min under the concentration range of 10-100 mg/L. Within the effective concentration range of 120-210 mg/L the fish reached stage 4 within 3 min, recovered within 10 min, and had a survival rate of 100% after immersed in the MS-222 solution for 15 min. After 5 min of immersing in MS-222 solution at 180 mg/L, the fish were taken out for air exposure, and the exposure time was designed for 0, 3 min, 6 min, 9min, 12 min, 15 min, 18 min and 21 min and then the fish were put into clean sea water. The recovery time was 100 s without showing any significance （P〉0.05）. Using another 5 fish to sink in MS-222 solution at 180 mg/L for 5 min, then exposing them to air for 3 min, and putting them back to the clean sea water, the same conduction was repeated one time daily over a period of 6 d. The recovery time was not significantly different, which indicated that the fish had no increased tolerance to MS-222 by repeated anaesthetization. The results indicate that MS-222 as an anaesthetic to adult Cynoglossus semilaevis GUnther is feasible in terms of the effects that fish can be induced anesthesia rapidly and recovery rapidly with a wide concentration range of safety without any increasing tolerance by repeated anaesthetization. [Journal of Fishery Sciences of China, 2008, 15 （1） ： 92-99]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第1期92-99,共8页
Journal of Fishery Sciences of China
基金
国家863计划项目(2005AA603110).
关键词
MS-222
麻醉
有效质量浓度
半滑舌鳎
MS-222
anaesthesia
effective concentration
Cynoglossus semilaevis Günther
