摘要
采用颜料法确定中华绒螯蟹(Eriocheir sinensis)循环血体积(V)与体质量(W)间的回归曲线方程为V=0.1576W-0.898(Rs=0.83,P<0.01),循环血体积与体质量的百分比(V/W)为(14.86±2.95)%。在确定中华绒螯蟹循环血体积的基础上,通过注射革兰氏阳性蜡状芽孢杆菌(Bacillus cereus)和革兰氏阴性大肠杆菌(Escherichiacoli),探讨进入中华绒螯蟹体内细菌随时间变化的清除规律以及通过组织学观察探讨细菌的清除方式。结果表明,中华绒螯蟹对两种细菌的清除作用相似,在注射菌后60min时对蜡状芽孢杆菌和大肠杆菌的细菌清除率都达到最高,分别为(88.57±4.84)%和(90.15±3.88)%;120min时对两种细菌清除率与60min时无显著性差异(P>0.05)。中华绒螯蟹的鳃和心脏中血细胞都可聚集而形成血细胞团,并具有对细菌的包裹和吞噬作用,血细胞团的形成以及包裹和吞噬作用是中华绒螯蟹清除细菌的重要途径。
Eriocheir sinensis is one of the main aquaculture species extensively cultured in China. It was reported that there were several immune factors in E. sinensis individually studied in vitro. Actually, the immune defense response to outside organisms, including bacteria clearance, is the cooperating result of all defense factors in vivo. In this study, the experiments were carded out by injecting different bacterium into the crab bodies, and the relationship of circulating haemolymph volume （V） and body weight （W） in crab bodies was determined. The regression equation was V ： 0.157 6W-0.898 （Rs= 0.83, P〈0.01） by the dye method of amaranth. The percentage of V/Win E. sinensis was （14.86 ± 2.95） % which was relatively low compared with that in other crab or shrimp species. On the basis of this circulating haemolymph volume in E. sinensis, the clearance rates with the lapse of time and the clearance ways on injected Bacillus cereus and Escherichia. coli in E. sinensis were studied by histological observation. The results showed that the clearance rates of B. cereus and E. coli by E. sinensis were （45.81 ± 13.12） % and （35.69 ± 15.19） % respectively 10 min after the bacteria injection, and reached the highest percentages 60 min after injection, which were （88.57 ±4.84） % and （90.15 ± 3.88） % respectively, and the clearance rates of bacteria at 120 min were not significantly different compared with those at 60 min after the injection （P〉0.05）. However, the clearance rates of B. cereus with the lapse of time in E.sinensis were lower than that in C. maenas. The differences of clearance rates between the same bacterium illustrated that the immune defense capabilities in different species of crabs were different. Meanwhile, haemocytes in gill started to form clumps at 10 min after the injection of bacteria, and had the abilities of encapsulation and phagocytosis of bacteria at 30 min, and were necrotic with broken bacteria at 120 min, all of which illustrated E. sinensis had non-specific immune functions such as forming clumps, encapsulation and phagocytosis. Non-specific immune functions are important ways of bacteria clearance in E. sinensis. [Journal of Fishery Sciences of China, 2008, 15 （1） ： 113-121 ]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第1期113-121,共9页
Journal of Fishery Sciences of China
基金
上海市重点学科建设项目(Y1101).
关键词
中华绒螯蟹
细菌清除率
包裹
吞噬
Eriocheir sinensis
bacterium clearance rate
encapsulation
phagocytosis
