摘要
本文在观察腹腔注射褪黑素(melatonin,MEL)拮抗吗啡依赖小鼠纳洛酮催促戒断反应的同时,采用放射免疫分析法、免疫组织化学法,结合计算机图像处理技术,测定其对小鼠中脑导水管周围灰质(periaqueductal grey,PAG)、下丘脑弓状核(hypothalamic arcuate nucleus,Arc)中β-内啡肽(β-endorphin,β-EP)含量的影响。结果表明,MEL(80 mg/Kg体重)显著抑制吗啡依赖小鼠戒断反应(P<0.05)的同时,可显著增加其中脑PAG中β-EP含量(p<0.05),减弱Arc中β-EP样免疫阳性反应强度(P<0.05)。上述结果提示,MEL可提高吗啡戒断小鼠中脑PAG中β-EP含量,降低Arc中β-EP含量。
The present study was undertaken to investigate the effect of melatonin on the content of β-endorphin （β-EP） in the hypothalamic arcuate nucleus （Arc） and periaqueductal grey （PAG） of midbrain in morphine withdrawal mice. Male Kunming mice were injected subcutaneously （s.c.） with an increasing dose of morphine continuously for 8 d to establish morphine dependence model. Withdrawal response was induced by naloxone （3 mg/kg body weight, s.c.）. The potency of withdrawal response was evaluated according to the jumping times and body weight loss. Ninty minutes prior to the precipitation of naloxone, 80 mg/kg body weight of melatonin （MEL） was injected intraperitoneally （i.p.） to observe its antagonistic effect on the withdrawal response in morphine-dependent mice. After behavioral observation, radioimmunoassay was used to determine the content of β-EP in the PAG of midbrain, and immunohistochemical assay was used to observe the intensity of β-EP-like immunoreactivity in the Arc in mice. It was shown that MEL inhibited the naloxone-precipitated withdrawal responses in mice significantly （P〈0.05）. In the meantime, MEL increased the content of β-EP in the PAG of midbrain significantly （P〈0.05） and attenuated the intensity of β-EP-like immunoreactivity in the Arc in mice （P〈0.05）. The results suggest that MEL increases the content of β-EP in the PAG of midbrain, decrease the content of β-EP in the Arc in morphine withdrawal mice.
出处
《生理学报》
CAS
CSCD
北大核心
2007年第6期765-769,共5页
Acta Physiologica Sinica
基金
This work was supported by the Project of Science and Technology Exploitation of Fujian Province
China(No.2005D073)
the Natural Science Foundation of Fujian Province
China(No.C0410018)
the Project of Science and Technology Bureau of Fujian Province
China(No.2006F5046)
关键词
褪黑素
吗啡依赖
戒断反应
下丘脑弓状核
中脑导水管周围灰质
β-内啡肽
melatonin
morphine dependence
withdrawal response
hypothalamic arcuate nucleus
periaqueductal grey of midbrain
β-endorphin
