摘要
目的检测乙肝患者血清中乙肝两对半(HBVM)、乙型肝炎病毒表面抗原大蛋白(HBV-LP)以及HBV DNA,比较在不同乙肝两对半模式的患者中HBV-LP与HBV DNA的检出率,探讨乙型肝炎病毒表面抗原大蛋白(HBV-LP)用于乙肝患者临床诊断的意义。方法采用酶联免疫吸附实验(ELISA)检测HBV-LP和乙肝两对半,采用荧光定量PCR方法对患者HBV DNA进行检测。结果(1)相同乙肝模式患者血清中HBV-LP与HBV DNA检出率差异无统计学意义;(2)143例小三阳乙肝患者血清中HBV-LP与HBV DNA阳性率差异无统计学意义,二者的检出一致率为82.52%[(65+53)/143];(3)HBV-LP吸光度(A值)与HBV DNA呈正相关关系(r=0.983)。结论乙型肝炎病毒表面抗原大蛋白与HBV DNA有良好的正相关关系,在HBeAg阴性的乙肝患者中乙肝病毒表面抗原大蛋白(HBV-LP)与HBV DNA有较高检出一致率,HBV-LP用于临床反映乙肝病毒复制水平,特别是HBeAg阴性乙肝患者体内病毒复制情况有重要的意义。
Objective To explore the clinical significance of HBV large protein （HBV-LP） in diagnosing viral replication, we detected the HBV-LP,HBV DNA and the hepatitis B viral markers （HBV M） in the serum of the patients infected with HBV.Methods HBV-LP and HBV M were analyzed by using ELISA. HBV-DNA was quantitatively detected using real-time fluorescent polymerase chain reaction. Results （1）No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the same HBV M （ P 〉 0.05）. （2） No significant difference between the positive rate of HBV DNA and HBV-LP was found HBeAg nagetive patients. （3） The contents of serum HBV-LP was positively correlated with the number of HBV DNA copies. Conclusion There was a close correlation between the positive rate of HBV-LP and HBV DNA, and HBV-LP is a reliable serological marker that can reflect the replication of HBV.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2007年第4期380-382,共3页
Chinese Journal of Experimental and Clinical Virology
