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蜡质芽孢杆菌酰基高丝氨酸内酯酶基因的克隆及序列分析 被引量：2

CLONING AND SEQUENCE ANALISIS OF AIIA GENE FROM BACILLUS CEREUS
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摘要 利用pMD18-T克隆载体从蜡质芽孢杆菌菌株T-HW 3中克隆了酰基高丝氨酸内酯酶基因(AHL-lactonase,aiiA)。测序结果表明,该基因(GenBank登录号为DQ000643)由753个碱基组成,编码含有250个氨基酸残基的蛋白质。该蛋白质的推测的分子量为28kDa,等电点为4.235左右。核苷酸序列的BLAST分析结果表明,与之同源性较高的基因均为蜡质芽孢杆菌组aiiA基因(86%-99%)。 A novel aiiA was cloned from Bacillus cereus T - HW3 by PCR with pMD18 - T vector and was sequenced. The nucleotide sequence had been registered in GenBank with accession No. 3. It consists of 753 bases which encoded a polypeptide of 250 amino acids residues with a calculated molecular weight of 28 kDa and an isoelectric point value of 4. 235. BLAST analysis of its nucleotide sequence showed 86% -99% identity with those of the aliA genes from B. cereus group.
作者 黄天培 潘洁茹 姚帆 黄张敏
机构地区 福建农林大学生物农药与化学生物学教育部重点实验室
出处 《武夷科学》 2007年第1期29-34,共6页 Wuyi Science Journal
基金 福建省青年科技人才创新项目(No.2006F3016) 福建省自然科学基金(B0610005)资助
关键词 蜡质芽孢杆菌 酰基高丝氨酸内酯酶基因 基因克隆 序列分析 Bacillus cereus aliA cloning sequence analysis
分类号 S476 [农业科学—农业昆虫与害虫防治] Q785 [生物学—分子生物学]
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参考文献21

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二级参考文献26

  • 1黄天培,杨梅,姚帆,黄张敏,俞晓敏,黄志鹏,黄必旺.蜡质芽孢杆菌aiiA基因的克隆及融合表达[J].福建农林大学学报（自然科学版）,2006,35(3):292-297. 被引量：9
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共引文献67

同被引文献43

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武夷科学
2007年 第1期

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